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Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP Grade

SKU:
13210-241GMP
Categories: , , ,

With quality standards built into the design and manufacturing process at every step, use our Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP to isolate high purity monocytes from elutriated leukapheresis material without the need for lysis or density gradient centrifugation.  Non-monocytes are targeted and removed with antibodies recognizing CD2, CD3, CD5, CD15, CD16, CD19, CD20, CD56, CD66b, CD123, and CD235ab via negative selection. Isolated monocytes are suitable for a wide variety of downstream assays, including differentiation, engineering, flow cytometry, and cell culture, or other functional studies. The Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP offers a capacity to isolate CD14+ monocytes from up to 5 x 109 total cells.

  • GMP grade – Our GMP grade allows you to have peace of mind, with stringent documentation and processes, traceability, and strict adherence to standards.
  • Higher Purity– Delivers a high purity population of human CD14+ monocytes that are healthy and ready for downstream use.
  • Shorter processing time – From leukopak bag to purified monocytes in under an hour enables greater productivity, adding flexibility to your day.
  • No dedicated equipment, no magnets, or columns – Only general tools and equipment are needed for our quick and easy workflow.

Request a Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP Grade Quote

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Product Details

The Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP streamlines CD14+ monocyte isolations by eliminating the need for excess equipment and time-consuming sample preparation (i.e., Ficoll gradients or lysis)—providing more time to spend focusing on other things that matter. By eliminating the need for pre-processing, the Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP minimizes handling steps and consistently delivers more isolated monocytes. Get the most out of your time and material with the Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP.

Mechanism of Action: Leukopak samples contain a milieu of immune cells (i.e., white blood cells), plasma, platelets, and red blood cells. The Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP uses a negative selection method to enrich monocytes by removing unwanted cell populations. These unwanted cells are labeled with biotin-CD2, CD3, CD5, CD15, CD16, CD19, CD20, CD56, CD66b, CD123, and CD235ab. BACS™ Streptavidin Microbubbles are mixed into the sample where they bind to the labeled cells and gently float them to the surface. Isolated are subsequently harvested.

How it Works: A biotin-antibody cocktail is mixed with the leukapheresis material to label unwanted cell populations. BACS™ Streptavidin Microbubbles are added and bind to the biotin-labeled cells. Due to their inherent buoyancy, the microbubbles quickly and gently float the non-monocytes to the surface. Following centrifugation, the monocytes are collected.

Additional Information:

  • Storage: 4°C
  • Cell Type: CD14+ monocytes
  • Sample Species and Source: Human Monocyte enriched elutriated Leukopak material
  • Cell Separation Method: Negative Selection
  • Capacity: 5 x 109 total cell (red blood cells and white blood cells combined)

This kit comes with:

  • BACS™ Streptavidin Microbubbles
  • Human CD14+ Monocyte Enriched Leukopak Biotin-Antibody Cocktail
  • FC Blocking Reagent
  • Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)

Recommended for Best Results

  • 20 rpm tube rotator for mixing (e.g., Thermo Scientific cat#: 88881002)
  • Centrifuge (swinging bucket rotor strongly preferred)
  • Vacuum aspirator

Key Features

The Human CD14+ Monocyte Enriched Leukopak Isolation Kit – GMP is designed to enrich monocytes from leukopaks. Akadeum’s novel Microbubbles enable processing, limiting the unnecessary loss of cells. The CD14+ monocyte isolation protocol is fast and gentle on cells, taking just one hour and not requiring upfront processing or magnets or columns. The result is a population of highly pure monocytes that are ready for downstream use.

Elevated Solutions for Sample Preparation

  • Ficoll- and lysis-free cell separation for the isolation of high purity
  • Fast and easy workflow; leukopak bag to cells in under 60 min.
  • Maximum yield of monocytes without compromising on purity.

Elegant in Simplicity, Powerful in Application

BACS™ Microbubble technology harnesses the power flotation to streamline your cell therapy workflow. Our novel technology couples performance, ease, and quality, bringing a new dimension to your cell culture.

Experience the Benefits of a Microbubble Workflow. Akadeum’s Microbubbles Are:

  • Gentle on cells: Harnessing the power of buoyancy, BACSTM Microbubbles swiftly and gently remove unwanted cells via flotation.
  • Fast, reliable, and scalable: The simple BACSTM Microbubbles protocol delivers reproducible results that can be easily scaled for greater numbers of cells or larger volumes.
  • Equipment-free: Free yourself from worrying about maintaining magnets, losing cells to clogged columns, or sacrificing space in the biosafety hood.

Looking for Answers?

At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and dedicate ourselves to helping researchers obtain exceptional results. If you’re interested in leveraging our BACS™ Microbubble platform to overcome existing hurdles in your cell isolation workflow, we want to hear from you! You can speak directly with an Akadeum scientist available to help you with an optimized workflow. Schedule a call today; we’d love to start a conversation!

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Data

Figure 1: Cell populations were defined using flow cytometry. Representative plots showing CD45+ cell staining for CD14 and CD16 for input material and post-separation material.

 

Figure 2: Monocyte concentration before and after isolation across 11 different donors showing high purity from a variety of input concentrations.

 

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