Product Details
With quality standards built into the design and manufacturing process at every step, Akadeum’s Human T Cell Depletion Kit – CR uses microbubbles to remove unedited T cells from CAR T cultures. Unwanted T cells expressing CD3 are positively isolated using a cocktail of biotinylated anti-CD3 antibodies, which are then captured using our buoyant Depletion Microbubbles that gently float to the top of the sample. This leaves a highly pure sample of engineered cells untouched and ready for downstream formulation. The highly scalable nature of this process means that it can easily be incorporated into a wide variety of cell therapy manufacturing workflows.
- Efficiently deplete CD-3 expressing cells quickly without losing precious engineered cells
- Flexibility to integrate into many existing workflows
- Increase recovery of gene-edited cells for downstream formulation
- Gentle process maintains the health of the cell therapy product
- Akadeum CR products are manufactured according to cGMP under a quality management system in compliance with 21 CFR 820, 211, and 210. They are developed following USP <1043> recommendations on ancillary materials and tested according to ISO 20399 and ISO 10993.
Mechanism of Action: When developing CAR T cell therapies, the ability to isolate pure populations of engineered cells after transduction is paramount. Cells still expressing CD3 can be harmful to the patients receiving these therapies. The Human T Cell Depletion Antibody Cocktail – CR recognizes the CD3 epitope using multiple, non-overlapping anti-CD3 clones for enhanced performance. CD3+ cells are labeled with antibodies, which are bound by the BACSTM Depletion Microbubbles – CR and removed from the sample using buoyancy. Unlabeled and successfully transduced CD3 negative cells are recovered by removal of microbubble-bound CD3+ cells, ready to use for downstream applications.
How it Works: Akadeum’s process offers an elegant solution for highly effective and scalable T cell depletion. Biotinylated anti-CD3 antibodies bind to untransduced T cells still expressing the CD3 epitope. Streptavidin coated Depletion Microbubbles – CR then selectively bind to the antibodies labeling the undesired cells. Due to the natural buoyancy of the microbubbles, they naturally float to the surface of the culture vessel pulling along CD3+ cells. The positive cells can then be easily extracted off the surface with the microbubbles leaving engineered cells untouched to settle to the bottom of the vessel.
Additional Information:
- Storage: 4°C
- Cell Type: CD3+ T Cells
- Sample Species and Source: Engineered Human CAR T cell cultures
- Cell Separation Method: Depletion
- Capacity: 5 x 109 total cells
This kit comes with:
- 10 mL BACSTM Depletion Microbubbles – CR
- 1 mL Human T Cell Depletion Antibody Cocktail – CR
Recommended for Best Results
- 20 rpm tube rotator for mixing (e.g., Thermo Scientific cat#: 88881002)
- Centrifuge (swinging bucket rotor strongly preferred)
- Vacuum aspirator
- 9-inch sterile Pasteur Pipets
- Sterile 5 mL / 50 mL tubes
- Buffer of choice: Phosphate-buffered saline (PBS, Ca2+ and Mg2+ free) supplemented with 0.5% serum and 2 mM EDTA, pH 7.2 is recommended.
