Product Details
The Akadeum Human Red Blood Cell Depletion Microbubbles consist of 1 ml of microbubbles with the capacity to deplete about 50 million human red blood cells.
Products
Human Red Blood Cell Depletion Microbubbles
$250.00
Why microbubbles?
- Up to 99 % capture and removal of RBCs
- 10 minute protocol
- No magnet
- Bubbles float while you are spinning your cells down
- A great alternative to additional lysis or Ficoll steps
Use microbubbles for
- Enrichment of circulating tumor cell or other rare cell populations
- Removal of contaminating RBCs after lysis
- Removal of contaminating RBCs after Ficoll or equivalent
What’s included
- Microbubbles targeted to red blood cells in 1–1.5 ml of reagent
- Enough microbubbles to remove approximately 50 million RBCs
- A simple and straightforward protocol
- Orders of 4 ml or more are discounted. Contact us at info@akadeum.com for more details
for more information and current discounts
Key Features
Akadeum’s Human Red Blood Cell Depletion Microbubbles are a microbubble-based cell isolation product designed to clean up samples with significant red blood cell (RBC) contamination. Common examples include RBCs that separate poorly in standard Ficoll or leukopheresis preparations. Other examples include bone marrow or dissociated tissue samples.
Red Blood Cell Depletion Microbubbles allow removal of unwanted erythrocytes when harsh lysis steps such as ammonium chloride must be avoided. This is a frequent concern when the RBC lysis conditions may activate, injure, or kill the cells of interest within a sample.
High Buoyancy
Upon engaging undesired RBCs, Akadeum microbubbles rapidly lift these cells to the top of the sample (typically less than 10 minutes for passive lifting in a microcentrifuge tube; less than 1 minute with centrifugation).
Excellent Specificity
Microbubbles employ a monoclonal murine IgG against the human RBC glycophorin-A sialoglycoprotein (CD235a). CD235a’s high specificity for erythrocytes, and its high density on RBC surfaces (10^6 per cell, or ~ 7,500 per square micron of RBC surface) make it an excellent targeting molecule for capture.
Fast, Easy Binding in Samples
In most samples of volume < 1 ml and total cell number < 10^8 total cells, mixing with RBC microbubbles can be completed in 1 minute. Subsequent centrifugation and RBC-bubble removal means the entire clean-up can be accomplished in less than 10 minutes.
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