Human Red Blood Cell Depletion Microbubbles

Key Features

Akadeum’s Human Red Blood Cell Depletion Microbubbles are a microbubble-based cell isolation product designed to clean up samples with significant red blood cell contamination. Common examples include RBCs that separate poorly in standard Ficoll or leukopheresis preparations. Other examples include bone marrow or dissociated tissue samples.

Human Red Blood Cell Depletion Microbubbles allow removal of unwanted erythrocytes when harsh lysis steps such as ammonium chloride must be avoided.  This is a frequent concern when the RBC lysis conditions may activate, injure, or kill the cells of interest within a sample.

High Buoyancy

Upon engaging undesired RBCs, Akadeum microbubbles rapidly lift these cells to the top of the sample (typically less than 10 minutes for passive lifting in a microcentrifuge tube, less than 1 minute with centrifugation).

Excellent Specificity

Microbubbles employ a monoclonal murine IgG against the human RBC glycophorin-A sialoglycoprotein (CD235a).  CD235a’s high specificity for erythrocytes and its high density on RBC surfaces (10^6 per cell, or ~ 7,500 per square micron of RBC surface) make it an excellent targeting molecule for capture.

Fast, Easy Binding in Samples

In most samples of volume < 1 mL and total cell number < 10^8 total cells, mixing with RBC microbubbles can be completed in 1 minute.  Subsequent centrifugation and RBC-bubble removal means the entire clean-up can be accomplished in less than 10 minutes.