Akadeum’s Human Red Blood Cell Depletion Microbubbles are designed to clean up samples with residual erythrocyte contamination, including human whole blood samples following RBC lysis or Ficoll isolation. Use of these microbubbles to remove residual RBC contamination prior to flow sorting can reduce final sort times by as much as 1/3, helping to isolate more cells for better science.
Mechanism of Action: Akadeum’s Human Red Blood Cell Depletion Microbubbles employ a monoclonal murine IgG against the human RBC glycophorin-A sialoglycoprotein (CD235a). CD235a’s high specificity for human erythrocytes, and its high density on RBC surfaces, make it an excellent affinity molecule for capture.
How it Works: Akadeum’s Human RBC Microbubbles are gently mixed into the sample where they bind to the contaminating RBCs. Using their inherent buoyancy, the microbubbles quickly and gently float the unwanted cells to the surface. An optional centrifugation step pellets the cells of interest, and the microbubbles, microbubble-bound RBCs, and supernatant are simply aspirated away leaving the untouched and enriched cells behind, happy, healthy, and ready for downstream use.
- Storage: Store microbubbles and Separation Buffer at 4°C
- Cell Type: Targeted removal of human RBCs
- Sample Species and Source: Human whole blood or bone marrow aspirate
- Cell Separation Method: Depletion
- Capacity: 1 mL of microbubbles to deplete up to 50 million human RBCs
This kit comes with:
- 1 mL Human RBC Depletion Microbubbles
- Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA, and 0.09% sodium azide)
For best results, the following supplies are recommended:
- Low retention wide orifice 200 uL pipet tips
- Centrifuge with swinging bucket rotor
- Vacuum aspirator