Requiring only 3 steps and 10 minutes, Akadeum’s exceptionally gentle Human Red Blood Cell (RBC) Depletion Microbubbles remove up to 99% of RBC contamination from biological samples while maintaining the health and physiology of delicate cells of interest.
1 mL of microbubbles with the capacity to deplete up to 50 million human RBCs
Fast & easy workflow effectively removes residual RBCs using 3-step, 10 min workflow
Reduce sort times by 1/3 while keeping cells happy and healthy
Akadeum’s Human Red Blood Cell Depletion Microbubbles are designed to clean up samples with residual erythrocyte contamination, including human whole blood samples following RBC lysis or Ficoll isolation. Use of these microbubbles to remove residual RBC contamination prior to flow sorting can reduce final sort times by as much as 1/3, helping to isolate more cells for better science.
Mechanism of Action:Akadeum’s Human Red Blood Cell Depletion Microbubbles employ a monoclonal murine IgG against the human RBC glycophorin-A sialoglycoprotein (CD235a). CD235a’s high specificity for human erythrocytes, and its high density on RBC surfaces, make it an excellent affinity molecule for capture.
How it Works: Akadeum’s Human RBC Microbubbles are gently mixed into the sample where they bind to the contaminating RBCs. Using their inherent buoyancy, the microbubbles quickly and gently float the unwanted cells to the surface. An optional centrifugation step pellets the cells of interest, and the microbubbles, microbubble-bound RBCs, and supernatant are simply aspirated away leaving the untouched and enriched cells behind, happy, healthy, and ready for downstream use.
Additional Information:
Storage: 4°C
Cell Type: Targeted removal of human RBCs
Sample Species and Source: Human whole blood or bone marrow aspirate
Cell Separation Method: Depletion
Capacity: 1 mL of microbubbles to deplete up to 50 million human RBCs
This kit comes with:
1 mL Human RBC Depletion Microbubbles
Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA, and 0.09% sodium azide)
For best results, the following supplies are recommended:
Low retention wide orifice 200 uL pipet tips
Centrifuge with swinging bucket rotor
Vacuum aspirator
Key Features
Buoyancy-activated cell sorting (BACS™) from Akadeum offers a novel way to target and remove contaminating red blood cells using science so simple, it floats! Our microbubbles combine the specificity of monoclonal antibodies with the power of gravity in a workflow that’s fast, easy, and effective while being gentle on cells. Requiring only 3 steps and 10 minutes, our Human RBC Depletion Kit removes up to 99% of RBCs while keeping delicate cells of interest happy and healthy. The entire process can be done directly in your sample vial without the need for additional equipment or expensive consumables, allowing you to greatly reduce sort times by 1/3 or more while ensuring a final enriched population of healthy, viable cells. And with a microbubble workflow, you eliminate the need to expose delicate cells to harsh chemicals or external forces.
Elevated Solutions for Sample Preparation:
Fast & easy microbubble workflow takes 10 minutes
Effective removal of contaminating RBCs
No magnet, no column, no harsh lysis buffers
Reduce sort times by 1/3+ while keeping cells happy
Elegant in Simplicity, Powerful in Application
Akadeum’s microbubble technology harnesses the power of buoyancy in a fast, easy and gentle workflow without the need for additional equipment. This unique, gentle approach maintains cell health and physiology.
No magnet. No column. Happy cells.
We’ve taken technology that traditionally requires additional equipment to accomplish targeted cell separation and put it into a single container where it’s self-separating. No harmful shear forces, no exposure to external forces like magnetic gradients. Stop losing your precious cells to harsh, outdated processing techniques.
Experience the benefits of a microbubble workflow. Akadeum’s Microbubbles Are:
Magnet-free, protecting your cells from harsh magnetic forces and eliminating the need for additional (and expensive!) equipment
Column-free, using microbubbles directly in the sample vial to separate cells allows for quick and easy isolation of any sample, any volume, anywhere
Gentle on cells, harnessing the targeted efficiency of affinity molecules coupled with the power of buoyancy to gently separate via flotation
Reliable and consistent, delivering fast and easy results that can be scaled for larger volumes with workflows that are compatible with nearly any downstream processing
Looking for Answers?
At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and are dedicated to helping the researchers we work with see exceptional results. If you’re interested in leveraging the microbubble platform to overcome existing hurdles in your sample preparation processes, we want to hear from you! Including any issues with red blood cells. You can speak directly to our team of scientists who are available to help you with an optimized workflow that takes advantage of our microbubble technology and solves long-standing headaches in sample preparation. Schedule a call today, we’d love to start a conversation!
Data
Using microbubbles from Akadeum to prepare a sample before beginning the cell sorting process can not only reduce sort times by 1/3 or more, but can result in a larger population of healthy, viable cells at the end of the sort. In the figure below, you can see the results of a PBMC sample before (left) and after (right) using Akadeum’s RBC Depletion Microbubbles.
In a real-world test of collecting 250,000 CD19+ B Cells from PBMCs, using Akadeum’s Human RBC Depletion Microbubble Kit created reduction in sort times of more than 33% each and every time.
Interested in learning more? Download our app note today:
Using microbubbles from Akadeum to prepare a sample before beginning a cell sorting process can greatly reduce sort times, using our simple workflow that takes 10 minutes, in any container, using any sample volume. No special equipment is necessary, no harsh forces are applied. Our microbubbles simply float the target to the surface for fast, easy, and gentle removal.
