Product Details
Overview
- Akadeum’s Human CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles to negatively select CD4+ T cells from peripheral blood mononuclear cells
- Non-CD4+ T cells (unwanted cells) are labeled with biotinylated antibodies and subsequently mixed with microbubbles
- Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet
- High purity human CD4+ T cells are left untouched and ready for downstream applications
Advantages:
- No column
- No magnet
- Up to 95% purity
- High yield
- Easy to use
- Fast
- Untouched and viable cells
Capacity: Full Kit = 1 billion cells; Trial Kit = 300 million cells
Components of Human CD4+ T Cell Isolation Kit (Full Kit #13210-130) (Trial Kit #23210-130):
- BACS™ Streptavidin Microbubbles
- Human CD4+ T Cell Antibody Cocktail (containing biotin-CD8, CD11B, CD14, CD16, CD19, CD36, CD56, CD123, CD235ab, and TCR-γδ)
- Separation buffer
Format:
- Antibody cocktail: Phosphate buffered solution containing 0.09% sodium azide
- Microbubbles: Aqueous solution
- Separation buffer: Phosphate buffered solution containing 0.5% BSA and 2 mm EDTA
Storage: Store all components at 4°C
Cell Type: CD4+ T cells
Sample Species and Source: Human PBMCs
Cell Separation Method: Negative selection
Area of Research: Immunology
Kit Type: T Cell Isolation Kit, CD4 T Cell Isolation Kit
