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Human T Cell Activation & Expansion Kit

Using BACS™ microbubble technology, the Human T Cell Activation & Expansion Kit was designed to isolate highly pure populations of T cells and activate them for use in cell therapy workflows. T cells are isolated, activated, and expanded using Akadeum’s Activation & Expansion Microbubbles in combination with an antibody cocktail that targets CD3 and CD28. This immensely scalable process yields the large numbers of highly activated and transducable cells needed to manufacture potent cell therapies. In addition, the gentleness of flotation-based culture results in fewer exhausted cells than legacy activation methods.

• Scalable to fit your cell therapy workflow needs.
• Closed-system in-bag workflows available.
• Reduced cellular exhaustion during T cell activation and expansion.
• Increased numbers of highly activated effector T cell populations.
• Industry-standard T cell expansion kinetics.

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Product Details

Akadeum’s Human T Cell Activation & Expansion Kit generates large numbers of highly activated, less exhausted cells, enabling better science and more effective cell therapies. Using BACS™ microbubble technology, the Human T Cell Activation & Expansion Kit was designed to isolate highly pure populations of T cells and activate them for use in cell therapy workflows. This highly scalable isolation, activation, and expansion process yields the large numbers of highly activated and transducable cells needed to manufacture potent cell therapies. In addition, the gentleness of flotation-based culture results in fewer exhausted cells than common activation methods. Akadeum’s novel approach to CAR-T cell production provides improvements over current industry standard methods, and the innate scalability of BACS™ microbubbles allows for rapid optimization of cell therapy workflows.

Mechanism of Action: A process that combines selection, activation, and expansion into a single workflow powered by BACS microbubbles is presented in this study. Due to the natural buoyancy of the microbubbles, activation and expansion take place at the top surface of the culture vessel. As the stimulated T cells divide, daughter cells dissociate from the microbubbles and settle at the bottom surface of the culture vessel where they are no longer in contact with the activation signals. This separation from the activation signals prevents overstimulation and results in a 2-fold lower expression of exhaustion markers relative to the industry-standard activation methods – only 25-30% PD-1+ T cell when microbubbles are used compared to over 90% when iron particle-based methods are used. The Human T Cell Activation & Expansion kit reduces the expression of exhaustion markers without compromising T cell activation as evidenced by the rapid upregulation of CD25 and other standard activation markers. Additionally, buoyant activation using microbubbles yield enhanced effector T cell populations and industry standard expansion kinetics.

How it Works: Starting from leukopak material or PBMCs, T cells are labeled with biotinylated antibodies targeting CD3 and CD28. BACS™ Activation & Expansion Microbubbles are mixed into the sample where they bind to the labeled cells and gently float them to the surface. Unwanted cells are removed from below the microbubble later, and the positively selected T cells are transferred to a culture vessel. As the cells divide daughter cells gently settle to the bottom of the plate, ready for collection.

Additional Information:

  • Storage: 4°C
  • Cell Type: T Cells
  • Sample Species and Source: Human PBMC or Leukopak material
  • Cell Separation Method: Positive Selection
  • Capacity: 800 x 106 nucleated cells

This kit comes with:

  • BACS™ Activation & Expansion Microbubbles
  • CD3/CD28 Activation & Expansion Antibody Cocktail

Recommended for Best Results

  • 20 rpm tube rotator for mixing (e.g., Thermo Scientific cat#: 88881002)
  • Centrifuge (swinging bucket rotor strongly preferred)
  • 9-inch sterile Pasteur Pipets
  • 5 mL / 50 mL tubes
  • Culture plates or bags

Key Features

Akadeum’s Human T Cell Activation & Expansion Kit generates large numbers of highly activated, less exhausted cells, enabling better science and more effective cell therapies. Using BACS™ microbubble technology, the Human T Cell Activation & Expansion Kit was designed to isolate highly pure populations of T cells and activate them for use in cell therapy workflows. Akadeum’s novel approach to CAR-T cell production provides improvements over current industry standard methods, and the innate scalability of BACS™ microbubbles allows for rapid optimization of cell therapy workflows.

Elegant in Simplicity, Powerful in Application

BACS™ Microbubble technology harnesses the power flotation to streamline your cell therapy workflow. Our novel technology couples performance, ease, and quality, bringing a new dimension to your cell culture.

What are the advantages of Akadeum’s BACSTM workflow?

  • Minimal expression of exhaustion markers
    • Microbubble enabled cultures result in significantly less expression of the exhaustion markers PD-1, TIM-3 and LAG-3 compared to magnetics.
  • No Compromise to expansion kinetics
    • The Akadeum Human T Cell Activation & Expansion kit  yields greater than 20-fold expansion over 10 days of culture.
  • Strong effector cytokine profile
    • Microbubble activation results in strong expression of effector cytokines.
  • Efficient for transduction, gentle on cells
    • T cells activated via microbubbles are highly transducible, while at the same time remaining healthier and less stressed.

Looking for Answers?

At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and dedicate ourselves to helping researchers obtain exceptional results. If you’re interested in leveraging our BACS™ Microbubble platform to overcome existing hurdles in your cell therapy workflow, we want to hear from you! You can speak directly with an Akadeum scientist available to help you with an optimized workflow. Schedule a call today; we’d love to start a conversation!

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Data

T cells were isolated from PBMCs using the Human T Cell Activation & Expansion Kit. Isolated microbubble-bound T cells were subsequently cultured for 13 days. Cell numbers and frequency of PD-1 expressing cells were determined at the indicated times.

 

Resources

Protocol: Coming soon!

View the Human T Cell Activation & Expansion Kit Flyer

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