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FACS vs. Flow Cytometry: Differences Between Flow Cytometry and FACS Cell Sorting

May 2021

FACS vs. Flow Cytometry As medical research becomes more advanced, scientists must use more complex technology to identify, separate, and prepare cells for downstream applications. Two popular methods for this include flow cytometry and fluorescence activated cell sorting. In the field of cell sorting and analysis, the terms flow cytometry and fluorescence activated cell sorting (FACS) are often used interchangeably. While each harness light scattering techniques and flowing cell samples …

CTC Separation: Circulating Tumor Cells Isolation Methods and CTC Isolation from Whole Blood

May 2021

CTC Separation When a cancerous tumor is present in the human body, some of the tumor cells can be shed into blood or lymph vessels, where they travel through the bloodstream alone or in clusters. These cells are called circulating tumor cells (CTCs). Scientists can collect these cells for research purposes through a process called CTC separation. CTC separation is the process of CTC isolation from whole blood samples. Residual …

The Simplest and Quickest Method for T Cell Isolation from Mouse Spleen

April 2021

Spleen Cell Isolation Just as a gold mine has different areas with higher concentrations of gold, different parts of the body have different levels of T cells. In a mouse, the spleen cell culture is composed of around 100 million splenocytes. Splenocytes are mononuclear white blood cells (WBCs) derived from or situated in the spleen. T cells typically comprise roughly 25% of the total splenocyte population. While murine (relating to …

CD4 Helper T Cells: What Are CD4 T Cells? CD4 T Cell Functions and Isolation Methods

April 2021

What are CD4 Cells? The human immune system is broken down into two major categories: the innate immune system and the adaptive immune system. The innate immune system is comprised of different physical, chemical, and cellular responses to pathogens that jump into action, offering general and non-specific first line defense when the immune system is called to act in an effort to contain the spread of pathogens. The adaptive immune …

Cell Separation Using Magnetic Beads: How Do Cell Separation Beads Work and How to Remove Magnetic Beads from Cells

April 2021

Cell Separation Using Magnetic Beads Due to a constant demand for enriched cell samples, there are a variety of methods used to isolate and extract target cells from a mixture. One of the more common techniques is magnetic-activated cell sorting (MACS). MACS uses magnetic beads and a magnetic separation column to perform cell enrichment. MACS The goal of MACS, also called immunomagnetic cell separation, is to isolate a single cell …

PBMC Isolation Protocol: Methods for the Isolation of White Blood Cells From Whole Blood

April 2021

Peripheral Blood Mononuclear Cells Within the human body, an interconnected system of cells works together to carry out a functional immune response. When presented with a harmful pathogen, a variety of lymphocytes and signaling molecules activate to combat and eradicate the threat. Scientists actively study this process to gain insight into possible routes for immunological research. One way to study the immune response is to look at peripheral blood mononuclear …

What Is PBMC? Human PBMC Cells, PBMC Composition, and Immune Cell Processing

March 2021

Peripheral Blood Mononuclear Cells PBMC stands for peripheral blood mononuclear cell. PBMCs are a variety of specialized immune cells that work together to protect our bodies from harmful pathogens. Their location in peripheral blood is significant because they act as a line of defense from infection and disease. PBMCs are typically collected by isolation from whole blood samples.  Where do PBMCs Come From? PBMCs are developed in the bone marrow …

What is Buffy Coat in Blood? Buffy Coat Preparation and Buffy Coat Cell Extraction

March 2021

What Is a Buffy Coat? In a sample of peripheral whole blood, less than 1% of the cells are white blood cells (WBCs) and platelets. When researchers put the sample through a centrifuge, a machine that spins the blood, those WBCs and platelets combine to form their own layer suspended between the red blood cells (RBCs) and supernatant plasma. This thin layer is called a buffy coat because of its …

Living Cells can React to Magnetic Signals

March 2021

Researchers and scientists around the world have long theorized that the inherent directional systems in various animals (including migratory animals like birds, butterflies, whales, and possibly even humans) could have a magnetic component – that internal navigational impulses are related in some way to Earth’s magnetic field. While this has been a long-standing theory, the underlying cellular mechanisms that support the ability of an organism to detect magnetic field to …

Cell Dissociation Methods for Disaggregation of Tissue: Mechanical vs Enzymatic vs Chemical

March 2021

Cell Dissociation Methods for Disaggregation of Tissue: Mechanical vs Enzymatic vs Chemical

Cell Dissociation Methods Research is undertaken step by step, and cutting corners provides inaccurate results. It’s important to follow proper protocol from beginning to end to ensure the highest quality findings possible. When working with cells of any kind (immune cells, tumor cells, etc.) the first step is always to collect the desired cell sample from the host tissue. Dissociation, sometimes called disaggregation, involves the breaking down of a cell …

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