How big are microbubbles?

November 2016 General

Microbubbles range in size from the nanometer length scale to micrometers. The choice in size has a lot to do with the application. For cell separation, it is important that the microbubbles are in something of a Goldilocks Zone, where they can float with a cell attached but not float too quickly. At Akadeum, we have chosen a size of microbubble that is specifically fine tuned for cell separation. In …

What is cell separation and cell sorting?

November 2016 General

We recognize that if you are reading this, you probably already understand or at least have a very good idea of what cell sorting and cell separation is. Still, you would be surprised by how many times we get asked questions like, “What is the difference between cell sorting and cell separation?” We also get questions like, “What are the different ways to separate cells?” or “What is the difference …

What are microbubbles made of?

October 2016 General

When we are out talking about Akadeum’s cell separation microbubbles, we come across a lot of people that think the microbubbles are purely made of a gas. While this is the common type of bubble that many of us are most familiar with, it is not the type of bubble used in separating cells. Akadeum’s microbubbles are made of thin shells of glass with a gaseous core. Microbubbles exist in many different …

Microbubbles and Centrifugation: How It Works

February 2016 Technical


Can microbubbles be pelleted by centrifugation? In short, no. At least not a pellet that you would see at the bottom of your tube when you spin down cells. Instead, what happens is anything with a density greater than water sinks and sinks faster when in a centrifuge. And anything with a density less than water rises and rises faster when in a centrifuge. How Microbubbles Float When Centrifuged So, …

How many cells can a microbubble lift?

February 2016 General

We hear this question all the time: How many cells can one of your microbubbles lift? For many users, the most important questions around microbubble-based separation have to do with how many microbubbles to add to a sample. Answering this question requires getting into the total “lifting capacity” of a microbubble. Part of the answer derives from how a microbubble engages cells, clumps of cells, or even larger structures such …

The Physics of How Microbubbles Sort Cells

January 2016 Technical


Flotation is the driving principle behind our cell sorting technology. It’s hard to imagine many other physical phenomena that humans have more of an intuition for than floating and sinking. The exact same rules of floating and sinking apply to a stem cell being lifted from a sample on the back of an Akadeum microbubble. In principal, it is as simple as pair of water wings that keeps a two-year-old …