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Self-Antigens vs. Non-Self Antigens: Examples & Cluster of Differentiation Markers

December 2021

Antigens are molecules present on the surface of cells that bind to receptors on antibodies or on the surface of lymphocytes. Antigens are classified based on where they originate, and the immune system discriminates between native and foreign antigens in order to fight against pathogens. Immunology research utilizes the binding properties of antigens with antibodies in order to detect, track, and isolate specific cell populations within blood samples. Self vs …

Achieve Gentle Mouse Naïve Cell Isolation Using Akadeum’s Microbubble Naïve Mouse Kits

December 2021

In working with biological samples for life science research, sample preparation is a critical first step to isolate the specific cells of interest from a complex mixture. This is especially true when looking to isolate rare cells of low abundance, and it is critically important when working with delicate cell types that the sample preparation method used for cell isolation is exceptionally gentle so that it does not interfere with …

Human RBC Depletion vs Mouse RBC Depletion: Differences in Protocols & Applications

November 2021

Sample preparation is often a critical first step in any number of life science workflows. Due to the inherent complexity within samples and diversity between applications, sample preparation needs can vary widely. One common first step is a “cleanup” step to deplete residual red blood cells (RBCs) from the sample before proceeding with downstream analysis. How Does RBC Depletion Work? Red blood cell depletion is a method of selection that …

High Throughput Human T Cell Enrichment for Immunological Research

November 2021

High Throughput Human T Cell Enrichment The field of immunological research is continuously expanding. More and more things are being discovered on a cellular and molecular level. Cell separation and enrichment has become an integral part of studying our immune cell behaviors. One of the most significant challenges in the research community has been finding a high throughput T cell enrichment method that can be used to isolate high volumes …

Gentle Mouse B Cell Enrichment: Strategies for Preserving Fragile Mouse B Cells

October 2021

Mouse B Cell Enrichment: How to Culture Isolated Mouse B Cells The primary function of B cells is antibody production. They are called B cells because they mature in the bone marrow, while T cells mature in the thymus. Receptors on the surface of a B cell bind to foreign antigens and develop antibodies that suppress that specific pathogen. Understanding more about how B cells work can provide insight into …

Necrosis Vs. Apoptosis: Processes, Necrotic Cell Death, & Apoptosis Steps

September 2021

The stages of cell death vary depending on the way in which a cell dies. When discussing cell death, there are two main types: apoptosis and necrosis. Each of these involves a unique process and has different effects on the rest of the body. What is Cell Apoptosis? Programmed cell death is called apoptosis, a regular growth, and development mechanism within an organism. This process is also known as “cellular …

Regulatory T Cells: Function of CD4, CD25 T Cells, and FOXP3 Treg Cells

September 2021

The human immune system is made up of many different cells–some of which are responsible for identifying unfamiliar pathogens, some of which recognize familiar pathogens, and some that attack anything harmful to the host. There are many moving parts that must cooperate to properly defend against foreign particles and diseases. These interactions between cells and pathogens are maintained and controlled by specialized lymphocytes called regulatory T cells. What Are Regulatory …

Using Akadeum’s Human CD4+ T Cell Isolation to Study T-Cell Immune Responses in the Pathogenesis of Human Hepatotropic Viruses

August 2021

Use of Akadeum’s gentle and effective Human CD4+ T Cell Isolation Kit results in a highly enriched sample of human CD4+ T cells, enabling detection and isolation of low-abundance, antigen-specific CD4+ T cells in patient samples. A research group led by Professor Georg Lauer at Massachusetts General Hospital and the Harvard Medical School recently used Akadeum’s Human CD4+ T Cell Isolation Kit to obtain high purity enrichment of viable, healthy …

FACS vs. Flow Cytometry: Differences Between Flow Cytometry and FACS Cell Sorting

May 2021

FACS vs. Flow Cytometry As medical research becomes more advanced, scientists must use more complex technology to identify, separate, and prepare cells for downstream applications. Two popular methods for this include flow cytometry and fluorescence activated cell sorting. In the field of cell sorting and analysis, the terms flow cytometry and fluorescence activated cell sorting (FACS) are often used interchangeably. While each harness light scattering techniques and flowing cell samples …

CTC Separation: Circulating Tumor Cells Isolation Methods and CTC Isolation from Whole Blood

May 2021

CTC Separation When a cancerous tumor is present in the human body, some of the tumor cells can be shed into blood or lymph vessels, where they travel through the bloodstream alone or in clusters. These cells are called circulating tumor cells (CTCs). Scientists can collect these cells for research purposes through a process called CTC separation. CTC separation is the process of CTC isolation from whole blood samples. Residual …

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