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Regulatory T Cells: Function of CD4, CD25 T Cells, and FOXP3 Treg Cells

September 2021

The human immune system is made up of many different cells–some of which are responsible for identifying unfamiliar pathogens, some of which recognize familiar pathogens, and some that attack anything harmful to the host. There are many moving parts that must cooperate to properly defend against foreign particles and diseases. These interactions between cells and pathogens are maintained and controlled by specialized lymphocytes called regulatory T cells. What Are Regulatory …

Using Akadeum’s Human CD4+ T Cell Isolation to Study T-Cell Immune Responses in the Pathogenesis of Human Hepatotropic Viruses

August 2021

Use of Akadeum’s gentle and effective Human CD4+ T Cell Isolation Kit results in a highly enriched sample of human CD4+ T cells, enabling detection and isolation of low-abundance, antigen-specific CD4+ T cells in patient samples. A research group led by Professor Georg Lauer at Massachusetts General Hospital and the Harvard Medical School recently used Akadeum’s Human CD4+ T Cell Isolation Kit to obtain high purity enrichment of viable, healthy …

Using Akadeum’s Mouse RBC Depletion Microbubbles to enrich splenocytes for iNKT isolation

July 2021

Use of Akadeum’s gentle and effective Mouse RBC Depletion Microbubbles results in a highly enriched sample of splenocytes for iNKT isolation while maintaining the health and physiology of these precious few available cells. Noemi Alejandra Saavedra-Avila, Ph.D., a Research Associate in the lab led by Steven Porcelli, M.D., at the Albert Einstein College of Medicine, used the Mouse RBC Depletion Kit from Akadeum Life Sciences in the hopes of finding …

FACS vs. Flow Cytometry: Differences Between Flow Cytometry and FACS Cell Sorting

May 2021

FACS vs. Flow Cytometry As medical research becomes more advanced, scientists must use more complex technology to identify, separate, and prepare cells for downstream applications. Two popular methods for this include flow cytometry and fluorescence activated cell sorting. In the field of cell sorting and analysis, the terms flow cytometry and fluorescence activated cell sorting (FACS) are often used interchangeably. While each harness light scattering techniques and flowing cell samples …

CTC Separation: Circulating Tumor Cells Isolation Methods and CTC Isolation from Whole Blood

May 2021

CTC Separation When a cancerous tumor is present in the human body, some of the tumor cells can be shed into blood or lymph vessels, where they travel through the bloodstream alone or in clusters. These cells are called circulating tumor cells (CTCs). Scientists can collect these cells for research purposes through a process called CTC separation. CTC separation is the process of CTC isolation from whole blood samples. Residual …

The Simplest and Quickest Method for T Cell Isolation from Mouse Spleen

April 2021

Spleen Cell Isolation Just as a gold mine has different areas with higher concentrations of gold, different parts of the body have different levels of T cells. In a mouse, the spleen cell culture is composed of around 100 million splenocytes. Splenocytes are mononuclear white blood cells (WBCs) derived from or situated in the spleen. T cells typically comprise roughly 25% of the total splenocyte population. While murine (relating to …

CD4 Helper T Cells: What Are CD4 T Cells? CD4 T Cell Functions and Isolation Methods

April 2021

What are CD4 Cells? The human immune system is broken down into two major categories: the innate immune system and the adaptive immune system. The innate immune system is comprised of different physical, chemical, and cellular responses to pathogens that jump into action, offering general and non-specific first line defense when the immune system is called to act in an effort to contain the spread of pathogens. The adaptive immune …

Cell Separation Using Magnetic Beads: How Do Cell Separation Beads Work and How to Remove Magnetic Beads from Cells

April 2021

Cell Separation Using Magnetic Beads Due to a constant demand for enriched cell samples, there are a variety of methods used to isolate and extract target cells from a mixture. One of the more common techniques is magnetic-activated cell sorting (MACS). MACS uses magnetic beads and a magnetic separation column to perform cell enrichment. MACS The goal of MACS, also called immunomagnetic cell separation, is to isolate a single cell …

PBMC Isolation Protocol: Methods for the Isolation of White Blood Cells From Whole Blood

April 2021

Peripheral Blood Mononuclear Cells Within the human body, an interconnected system of cells works together to carry out a functional immune response. When presented with a harmful pathogen, a variety of lymphocytes and signaling molecules activate to combat and eradicate the threat. Scientists actively study this process to gain insight into possible routes for immunological research. One way to study the immune response is to look at peripheral blood mononuclear …

What Is PBMC? Human PBMC Cells, PBMC Composition, and Immune Cell Processing

March 2021

Peripheral Blood Mononuclear Cells PBMC stands for peripheral blood mononuclear cell. PBMCs are a variety of specialized immune cells that work together to protect our bodies from harmful pathogens. Their location in peripheral blood is significant because they act as a line of defense from infection and disease. PBMCs are typically collected by isolation from whole blood samples.  Where do PBMCs Come From? PBMCs are developed in the bone marrow …

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