Akadeum Is Joining the Fight Against COVID-19

April 2020


The coronavirus pandemic has highlighted a need for fast and accurate methods for identifying RNA viruses through nucleic acid capture and detection. Akadeum Life Sciences is up to the challenge. In a relatively short time, the coronavirus pandemic has spread across the globe. This novel virus was first noted in Wuhan, China in December 2019 and arrived in the United States in January 2020. The World Health Organization (WHO) called …

What is FACS?

March 2020


Fluorescence-activated cell sorting (FACS), sometimes called fluorescence-assisted cell sorting, is a specialized type of flow cytometry that uses fluorescent markers to target and isolate cell groups. It is commonly used in hematopoiesis, oncology, and stem cell biology research.

What is the difference between positive and negative cell separation?

March 2020


Cell isolation methods and technologies use either a positive or negative cell separation approach. Here are some of the differences when considering a cell separation approach:

How to Isolate Blood Cells from Plasma

March 2020


Blood separation is a key part of many life sciences. Researchers in clinical labs and other biotechnical roles commonly analyze blood cells, but to perform this research, whole blood must first be separated into its components. By isolating and enriching target cells from plasma, scientists can then detect diseases, study immunological functions, and further process specific cell types. Components of Whole Blood Whole blood consists of red cells, white cells, …

What is cell isolation?

March 2020


Cell isolation—also referred to as cell separation or cell sorting—is the process of isolating one cell population from other cells in a heterogeneous biological sample. Targeted cells are identified, isolated, and then separated according to their type.

How big are microbubbles?

November 2016


Microbubbles range in size from the nanometer length scale to micrometers. The choice in size has a lot to do with the application. For cell separation, it is important that the microbubbles are in something of a Goldilocks Zone, where they can float with a cell attached but not float too quickly. At Akadeum, we have chosen a size of microbubble that is specifically fine tuned for cell separation. In …

What is cell separation and cell sorting?

November 2016


We recognize that if you are reading this, you probably already understand or at least have a very good idea of what cell sorting and cell separation is. Still, you would be surprised by how many times we get asked questions like, “What is the difference between cell sorting and cell separation?” We also get questions like, “What are the different ways to separate cells?” or “What is the difference …

What are microbubbles made of?

October 2016


When we are out talking about Akadeum’s cell separation microbubbles, we come across a lot of people that think the microbubbles are purely made of a gas. While this is the common type of bubble that many of us are most familiar with, it is not the type of bubble used in separating cells. Glass Shells with a Gaseous Core Akadeum’s microbubbles are made of thin shells of glass with a …

Microbubbles and Centrifugation: How It Works

February 2016


Can microbubbles be pelleted by centrifugation? In short, no. At least not a pellet that you would see at the bottom of your tube when you spin down cells. Instead, what happens is anything with a density greater than water sinks and sinks faster when in a centrifuge. And anything with a density less than water rises and rises faster when in a centrifuge. How Microbubbles Float When Centrifuged So, …

How many cells can a microbubble lift?

February 2016


We hear this question all the time: How many cells can one of your microbubbles lift? For many users, the most important questions around microbubble-based separation have to do with how many microbubbles to add to a sample. Answering this question requires getting into the total “lifting capacity” of a microbubble. Part of the answer derives from how a microbubble engages cells, clumps of cells, or even larger structures such …