Products

Mouse T Cell Isolation Kit

$590.00

  • Akadeum’s Mouse T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles and biotinylated antibodies to enrich T cells from splenocytes.
  • Negative selection enrichment method
  • Process 10 samples, up to 100  million cells per sample
SKU: 10020 Category:

Product Details

Overview

  • Akadeum’s Mouse T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles to negatively select T cells from splenocytes.
  • Non-T cells (unwanted cells) are labeled with biotinylated antibodies and subsequently mixed with microbubbles.  
  • Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet.
  • High purity mouse T cells are left untouched and ready for downstream applications.

Advantages:

  • No Column
  • No Magnet
  • Up to 96% purity
  • High Yield
  • Easy to use
  • Fast
  • Untouched and viable cells

Capacity: Kit for 109 total cells

Components of Mouse T Cell Isolation Kit (Product # 10020):

  • BACS Streptavidin Microbubbles
  • Mouse T Cell Antibody Cocktail (containing biotin-B220 (CD45R), CD11b, CD11c, CD24, CD49b, Gr1, TER119)
  • Separation Buffer

Format:

  • Antibody cocktail: Phosphate buffered solution containing 0.09% sodium azide
  • Microbubbles: Aqueous solution
  • Separation buffer: Phosphate buffered solution containing 0.5% BSA and 2 mM EDTA
  • Storage: Store all components at 4°C

Cell Type: Naive / resting T cells

Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged. Other mouse starting materials have not been evaluated by Akadeum and may require additional user optimization.

Cell Separation Method: Negative Selection

Area of Research: Immunology

Data

Microbubbles were used to enrich mouse splenocytes to a purity of 96.9 % from a 40.1 % starting purity  (shown below).mouse t cell enrichment flow data

Resources

Mouse T Cell Isolation Kit Product Info Sheet