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Mouse Naïve CD4+ T Cell Isolation Kit

$320.00$640.00

  • Akadeum’s Mouse Naïve CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles to negatively select naïve CD4+ T cells from mouse splenocytes.
  • Negative selection enrichment method
  • With the Full Kit, process 10 samples, up to 100 million cells per sample
  • With the Trial Kit, process 3 samples, up to 100 million cells per sample
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Product Details

Overview

  • Akadeum’s Mouse Naïve CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles to negatively select naïve CD4+ T cells from mouse splenocytes.
  • Non-naïve CD4+ T cells (unwanted cells) are labelled with biotinylated antibodies targeting B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD25, CD44, CD49b, Gr-1, TCR-y8 and TER-119, and subsequently
    mixed with microbubbles.
  • Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet.
  • High purity mouse naïve CD4+ T cells are left untouched and ready for downstream applications.

Advantages:

  • No column
  • No magnet
  • Up to 96% purity
  • High yield
  • Easy to use
  • Fast
  • Untouched and viable cells

Capacity: Full Kit = 1 billion cells; Trial Kit = 300 million cells

Components of Mouse Naïve CD4+ T Cell Isolation Kit (#12210-150; Trial Kit #22210-150):

  • BACS Streptavidin Microbubbles
  • Mouse naïve CD4+ T cell biotin-antibody cocktail
  • Separation buffer

Storage: Store microbubbles, antibody cocktail, and Separation Buffer at 4°C

Cell Type: Naive T cells

Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged.

Cell Separation Method: Negative selection

Area of Research: Immunology

Kit Type: T Cell Isolation Kit, CD4 T Cell Isolation Kit

Additional information

Cell Type

Data

Example of Separation Using Akadeum Mouse Naïve CD4+ T Cell Isolation Kit

Fresh murine naïve CD4+ T cells were isolated from a heterogenous splenocyte population. Dead cells and debris were excluded from analysis and naïve CD4+ T cells were labeled with CD62L-APC and CD4-APC Fire 750 (shown below).

Resources

Mouse Naïve CD4+ T Cell Isolation Kit Product Info Sheet

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