Akadeum’s Mouse Naïve CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable naïve CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.
This kit is designed to process 10 samples, up to 100 million cells per sample
Fast & easy workflow enriches mouse splenocytes for naïve CD4+ T cells in 30 minutes
No magnet, no column, no harsh chemicals
Microbubble enrichment maintains cell health and physiology
Akadeum’s Mouse Naïve CD4+ T Cell Isolation Kit delivers a high-purity population of mouse naïve CD4+ T cells that are ready for downstream use. Use of these microbubbles to enrich mouse splenocyte samples for naïve CD4+ T cells prior to flow sorting can greatly reduce final sort times, helping to isolate more cells for better science. Akadeum’s microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of immune cells during enrichment.
Mechanism of Action:Akadeum’s Mouse Naïve CD4+ T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the naïve CD4+ T cells of interest while maintaining cellular physiology. The undesired (non-naïve T) cells are labeled with Akadeum’s optimized cocktail of biotinylated antibodies targeting B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD25, CD44, CD49b, Gr-1, TCR-γδ and TER-119. Streptavidin-coated microbubbles are then mixed into the sample, binding to the labeled cells and floating them to the top of the sample container for removal.
How it Works: First, Akadeum’s biotinylated antibody cocktail is added to the sample to label the contaminating cells. Next, the microbubbles are mixed into the sample where the seek out and bind to the unwanted cells. Once bound, they quickly and gently float the undesired cells to the surface. An optional centrifugation step pellets the naïve CD4+ T cells, and the microbubbles, microbubble-bound cells, and supernatant are easily aspirated away leaving the untouched and enriched naïve CD4+ T cells happy, healthy, and ready for downstream use.
Additional Information:
Storage: Store microbubbles, antibody cocktail, and Separation Buffer at 4°C
Cell Type: Naïve T cells
Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged.
Cell Separation Method: Negative Selection
Capacity: 1 billion cells
This kit comes with:
Akadeum’s BACS™ Streptavidin Microbubbles
Akadeum’s Mouse Naïve CD4+ T Cell Antibody Cocktail
Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA, and 0.09% sodium azide)
5mL Eppendorf Tubes
For best results, the following supplies are recommended:
Low retention 1mL pipet tips
Centrifuge with swinging bucket rotor
Vacuum aspirator
Key Features
Akadeum’s Mouse Naïve CD4+ T Cell Isolation Kit is fast, easy, and exceptionally gentle on delicate and rare immune cells. We understand how challenging it can be to obtain a large enough population of healthy cells of low abundance, and our naïve CD4+ T cell enrichment is specifically designed for fast and gentle sample preparation. Using microbubbles from Akadeum to enrich the sample prior to cell sorting can not only greatly reduce sort times, but can result in a larger population of healthy, viable cells. Our simple workflow takes only 30 minutes and can be performed directly in the sample container, no specialized equipment needed and no harsh forces or unnecessary chemicals applied. Our microbubbles simply float the contaminating cells to the surface for removal, leaving the naïve CD4+ T cells untouched and ready for use.
Elevated Solutions for Sample Preparation:
Gentle enrichment for naïve CD4+ T cells
Maintains health and viability of target cells
No additional equipment (no magnets, no columns)
30-minute microbubble protocol is fast and easy
Elegant in Simplicity, Powerful in Application
Akadeum’s microbubble technology harnesses the power of buoyancy in a fast, easy and gentle workflow without the need for additional equipment. This unique, gentle approach maintains cell health and physiology.
No magnet. No column. Happy cells.
We’ve taken technology that traditionally requires additional equipment to accomplish targeted cell separation and put it into a single container where it’s self-separating. No harmful shear forces, no exposure to external forces like magnetic gradients. Stop losing your precious cells to harsh, outdated processing techniques.
Experience the benefits of a microbubble workflow. Akadeum’s Microbubbles Are:
Magnet-free, protecting your cells from harsh magnetic forces and eliminating the need for additional (and expensive!) equipment
Column-free, using microbubbles directly in the sample vial to separate cells allows for quick and easy isolation of any sample, any volume, anywhere
Gentle on cells, harnessing the targeted efficiency of affinity molecules coupled with the power of buoyancy to gently separate via flotation
Reliable and consistent, delivering fast and easy results that can be scaled for larger volumes with workflows that are compatible with nearly any downstream processing
Looking for Answers?
At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and are dedicated to helping the researchers we work with see exceptional results. If you’re interested in leveraging the microbubble platform to overcome existing hurdles in your sample preparation processes, we want to hear from you! You can speak directly to our team of scientists who are available to help you with an optimized workflow that takes advantage of our microbubble technology and solves long-standing headaches in sample preparation. Schedule a call today, we’d love to start a conversation!
Data
Fresh murine naïve CD4+ T cells were isolated from a heterogenous splenocyte population. Dead cells and debris were excluded from analysis and naïve CD4+ T cells were labeled with CD62L-APC and CD4-APC Fire 750.
