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Human T Cell Leukopak Isolation Kit

Isolate truly untouched T cells directly from leukapheresis material without the need for lysis or density gradient centrifugation. The Human T Cell Leukopak Isolation Kit was developed with BACS™ Microbubbles to isolate pristine and untouched T cells from leukopak material. Non-T cells are targeted and removed with antibodies recognizing CD14, CD16, CD19, CD20, CD36, CD56, CD123, CD235ab via negative selection. Isolated T cells are suitable for flow cytometry, molecular assays, activation and expansion, cell culture, or other functional studies. The Human T Cell Leukopak Isolation Kit has the capacity to isolate T cells from an average half-leukopak (12.5 x 109 total cells).

  • Chemical-free processing that avoids exposing cells to lysis and density gradient reagents for uncovering more meaningful biology
  • From leukopak bag to purified cells in under an hour enables productivity and increased scientific discovery
  • Industry leading recovery of T cells allows you to get the most out of your leukopaks

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Product Details

The Human T Cell Leukopak Isolation Kit streamlines T cell isolations by eliminating the need for excess equipment and time-consuming sample preparation (i.e., Ficoll gradients or lysis) – allowing you more time to spend focusing on what matters, your science. By eliminating the need for pre-processing, the Human T Cell Leukopak Isolation Kit minimizes handling steps and consistently delivers more isolated T cells. Get the most out of your time and material with the Human T Cell Leukopak Isolation Kit.

Mechanism of Action: Leukopak samples contain a milieu of immune cells (i.e., white blood cells), plasma, platelets, and red blood cells. TheHuman T Cell Leukopak Isolation Kit uses a negative selection method to enrich T cells by removing unwanted cell populations. These unwanted cells are labeled with biotin-CD14, CD16, CD19, CD20, CD36, CD56, CD123, CD235ab. BACS™ Streptavidin Microbubbles are mixed into the sample where they bind to the labeled cells and gently float them to the surface. Untouched T cells in their original state are subsequently harvested.

How it Works: The Human T Cell from Leukopak Biotin- Antibody Cocktail is added to the leukapheresis product to label unwanted cell populations. BACS™ Streptavidin Microbubbles are added to the sample and bind to the biotin-labeled cells. Due to their inherent buoyancy, the microbubbles quickly and gently float the non-T cells to the surface. Following centrifugation, the T cells are collected.

Additional Information:

  • Storage: 4°C
  • Cell Type: T Cells
  • Sample Species and Source: Human Leukopaks
  • Cell Separation Method: Negative Selection
  • Capacity: 12.5 x 10⁹ total cells (enough to process an average half-leukopak)

This kit comes with:

  • BACS™ Streptavidin Microbubbles
  • Human T Cell from Leukopak Biotin-Antibody Cocktail
  • Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
  • 50 mL Separation Tubes

Recommended for Best Results

  • 20 rpm tube rotator for mixing (e.g., Thermo Scientific cat#: 88881002)
  • Centrifuge (swinging bucket rotor strongly preferred)
  • Vacuum aspirator

Key Features

The Human T Cell Leukopak Isolation Kit is designed to enrich T Cells from leukopaks. Akadeum’s novel Buoyancy Activated Cell Sorting (BACS™) Microbubbles cut handling steps, limiting unnecessary loss of cells. The T cell isolation protocol is fast and gentle on cells, taking just one hour and not requiring upfront processing or magnets or columns. The result is a population of pristine and highly pure T cells that are ready for downstream use.

Elevated Solutions for Sample Preparation

  • Ficoll- and lysis-free cell separation for the isolation of truly untouched T cells.
  • Fast and easy workflow; leukopak bag to cells in under 60 min.
  • Maximum yield of T cells without compromising on high purity.

Elegant in Simplicity, Powerful in Application

BACS™ Microbubble technology harnesses the power of flotation to provide a fast, easy, and gentle T cell isolation without the need for pre-processing of the leukopak material. Our novel technology couples performance, ease, and quality, bringing cell separation to the next level.

Experience the benefits of a microbubble workflow. Akadeum’s microbubbles are:

  • Equipment-free: Free yourself from worrying about the maintaining magnets, losing cells to clogged columns, or sacrificing space in the biosafety hood.
  • Gentle on cells: Harnessing the power of buoyancy, BACSTM Microbubbles swiftly and gently remove unwanted cells via flotation.
  • Fast, reliable, and scalable: The simple BACSTM Microbubbles protocol delivers reproducible results that can be easily scaled for greater numbers of samples or larger volumes.

Looking for Answers?

At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and dedicate ourselves to helping researchers obtain exceptional results. If you’re interested in leveraging our BACS™ Microbubble platform to overcome existing hurdles in your cell isolation workflow, we want to hear from you! You can speak directly with an Akadeum scientist available to help you with an optimized workflow. Schedule a call today; we’d love to start a conversation!

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Untouched T cells were isolated from 3 x 109 total leukopak cells using the Human T Cell Leukopak Isolation Kit. Isolated T cells were labeled with CD45-FITC and CD3-APC Fire/750. The fluorescently labeled cells were analyzed by flow cytometry.

Leukopak Human T Cell Isolation Kit Plots



View The Leukopak H2H App Note 

View the Human T Cell Leukopak Isolation Protocol
View the Human T Cell Leukopak Isolation Kit (Closed System) User Guide

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