The Human B Cell Isolation Kit delivers a high purity population of human B cells that is ready for downstream use. The BACS™ Microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of immune cells during enrichment.
Mechanism of Action: The Human B Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich B cells. Non-B cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD2, CD3, CD14, CD15, CD16, CD36, CD43, CD56, CD57, CD123, CD161, CD235ab, and CD314.
How it Works: The biotinylated antibody cocktail is added to the sample to label unwanted cells. BACS™ Microbubbles are added to the sample and bind to the antibody-labeled cells. Microbubbles bound to the undesired cells, quickly and gently float the undesired cells to the surface. B cells, the cells of interest, are pelleted through centrifugation. Microbubble-bound cells and supernatant are easily aspirated away, leaving untouched and enriched B cells healthy and ready for downstream use.
- Storage: 4°C
- Cell Type: Human B Cells
- Sample Species and Source: Human MNCs
- Cell Separation Method: Negative Selection
- Capacity: 1 billion MNCs
This kit comes with:
- BACS™ Streptavidin Microbubbles
- Human B Cell Biotin Antibody Cocktail
- Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, and 0.5% biotin-free BSA)
- 5 mL Tubes
For best results, the following supplies are recommended:
- 20 rpm tube rotator for mixing
- Centrifuge with swinging bucket rotor
- Vacuum aspirator
- 30 μm cell strainer