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Human PBMC Leukopak Isolation Kit

SKU:
13210-223 Human PBMC Leukopak Isolation Kit
13210-223T Human PBMC Leukopak Isolation Kit - Research Scale Categories: , ,

Isolate truly untouched Human Leukopak Peripheral Blood Mononuclear Cells (PBMCs) directly from leukapheresis material without the need for lysis or density gradient centrifugation. The Human PBMC Leukopak Isolation Kit was developed with BACS™ Microbubbles to isolate untouched Leukopak PBMCs from leukapheresis material. Non-PBMCs are targeted and removed with antibodies recognizing CD66b, CD123, CD235ab via negative selection. Isolated Leukopak PBMCs are suitable for flow cytometry, molecular assays, activation and expansion, cell culture, and other functional studies. The Human PBMC Leukopak Isolation Kit has the capacity to isolate PBMCs from an average half-leukopak (12.5 x 109 total cells, red blood cells and white blood cells combined).

The Human PBMC Leukopak Isolation Kit has the capacity to isolate PBMCs from 12.5 x 109 total cells (an average half-leukopak, red blood cells and white blood cells combined).

The Human PBMC Leukopak Isolation Kit, Research Scale has the capacity to isolate PBMCs from 2.0 x 109 total cells red blood cells and white blood cells combined).

  • From leukopak bag to purified PBMCs in under an hour enables productivity and increased scientific discovery
  • Industry-leading recovery of PBMCs allows you to get the most out of your leukopaks
  • Chemical-free processing that avoids exposing cells to lysis and density gradient reagents for uncovering more meaningful biology

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Product Details

The Human PBMC Leukopak Isolation Kit streamlines PBMC isolations by eliminating the need for excess equipment and time-consuming sample preparation (i.e., Ficoll gradients or lysis)—allowing you more time to spend focusing on what matters, your science. By ruling out the need for pre-processing, the Human PBMC Leukopak Isolation Kit minimizes handling steps and consistently delivers more isolated leukopak PBMCs. Get the most out of your time and material with the Human PBMC Leukopak Isolation Kit.

Mechanism of Action: Leukopak samples contain a milieu of immune cells (i.e., white blood cells), plasma, platelets, and red blood cells. The Human PBMC Leukopak Isolation Kit uses a negative selection method to enrich leukopak PBMCs by removing unwanted cell populations.

These unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD66b, CD123, and CD235ab. BACS™ Streptavidin Microbubbles are mixed into the sample where they bind to the labeled cells and gently float them to the surface. Untouched leukopak PBMCs in their original state are subsequently harvested. Isolated leukopak PBMCs are suitable for flow cytometry, molecular assays, activation and expansion, cell culture, or other functional studies.

How it Works: The Human PBMC Cell from Leukopak Biotin-Antibody Cocktail is added to the leukapheresis product to label unwanted cell populations. BACS™ Streptavidin Microbubbles are added to the sample and bind to the biotin-labeled cells. Due to their inherent buoyancy, the microbubbles quickly and gently float the non-T cells to the surface. Following centrifugation, the T cells are collected.

Additional Information:

  • Storage: 4°C
  • Cell Type: PBMCs
  • Sample Species and Source: Human Leukopaks (red blood cells and white blood cells combined)
  • Cell Separation Method: Negative Selection
  • Capacity:
  • Human PBMC Leukopak Isolation Kit: 12.5 x 109 total cells (enough to process an average half-leukopak)
  • Human PBMC Leukopak Isolation Kit, Research Scale: 2.0 x 109 total cells
  • Research Use Only

This kit comes with:

Human PBMC Leukopak Isolation Kit:

  • 62.5 mL BACS™ Streptavidin Microbubbles
  • 12.5 mL Human PBMC from Leukopak Biotin-Antibody Cocktail
  • 500 mL Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
  • 3 Microbubble Separation Tubes

Human PBMC Leukopak Isolation Kit, Research Scale:

  • 20 mL BACS™ Streptavidin Microbubbles
  • 2 mL Human PBMC from Leukopak Biotin-Antibody Cocktail
  • 200 mL Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)

Recommended for Best Results

  • 20 rpm tube rotator for mixing (e.g., Thermo Scientific cat#: 88881002)
  • Centrifuge (swinging bucket rotor strongly preferred)
  • Vacuum aspirator
  • 50 mL centrifuge tubes

Key Features

The Direct from Human PBMC Leukopak Isolation Kit is designed to enrich PBMCs from leukopaks. Akadeum’s novel Buoyancy Activated Cell Sorting (BACS™) Microbubbles enable processing, limiting the unnecessary loss of cells. The PBMC isolation protocol is fast and gentle on cells, taking just one hour and not requiring upfront processing or magnets or columns. The result is a population of pristine and highly pure PBMCs that are ready for downstream use.

Elevated Solutions for Sample Preparation

  • Say goodbye to lysis and ficoll – Chemical-free processing that avoids exposing cells to lysis and density gradient reagents for truly untouched PBMCs.
  • Enjoy higher recovery – Industry leading recovery of PBMCs allows you to get the most out of your leukopaks, limiting donor recalls.
  • Scalable to your needs – Process more material per isolation and more isolations per shift.
  • Gentle on fragile cells – Exceptionally gentle on delicate PBMC isolation.
  • Shorter processing time – From leukopak bag to purified cells in under an hour enables greater productivity, adding flexibility to your day.
  • Painless platelet removal – Spare your cells from repeated washes and centrifugations.

Elegant in Simplicity, Powerful in Application

BACS™ Microbubble technology harnesses the power flotation to provide a fast, easy, and gentle T cell isolation without the need for pre-processing of the leukopak material. Our novel technology couples performance, ease, and quality, bringing cell separation to the next level.

Experience the Benefits of a Microbubble Workflow. Akadeum’s Microbubbles Are:

  • Equipment-free: Free yourself from worrying about maintaining magnets, losing cells to clogged columns, or sacrificing space in the biosafety hood.
  • Gentle on cells: Harnessing the power of buoyancy, BACSTM Microbubbles swiftly and gently remove unwanted cells via flotation.
  • Fast, reliable, and scalable: The simple BACSTM Microbubbles protocol delivers reproducible results that can be easily scaled for greater numbers of samples or larger volumes.

Looking for Answers?

At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and dedicate ourselves to helping researchers obtain exceptional results. If you’re interested in leveraging our BACS™ Microbubble platform to overcome existing hurdles in your cell isolation workflow, we want to hear from you! You can speak directly with an Akadeum scientist available to help you with an optimized workflow. Schedule a call today; we’d love to start a conversation!

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Data

Isolated PBMCs were labeled for CD45 and CD235a. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells and debris were excluded from the analysis.

 

Resources

Human PBMC Leukopak Isolation Kit

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