Akadeum’s Human T Cell Isolation Kit delivers a high-purity population of human T cells that are ready for downstream use. Use of these microbubbles to enrich human PBMC samples for T cells prior to flow sorting can greatly reduce final sort times, helping to isolate more cells for better science. Akadeum’s microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of immune cells during enrichment.
Mechanism of Action:Akadeum’s Human T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the T cells of interest while maintaining cellular physiology. The undesired (non-T) cells are labeled with Akadeum’s optimized cocktail of biotinylated antibodies targeting CD14, CD16, CD19, CD36, CD56, CD123, and CD235a. Streptavidin-coated microbubbles are then mixed into the sample, binding to the labeled cells and floating them to the top of the sample container for removal.
How it Works: First, Akadeum’s biotinylated antibody cocktail is added to the sample to label the contaminating cells. Next, the microbubbles are mixed into the sample where the seek out and bind to the unwanted cells. Once bound, they quickly and gently float the undesired cells to the surface. An optional centrifugation step pellets the T cells, and the microbubbles, microbubble-bound cells, and supernatant are easily aspirated away leaving the untouched and enriched T cells happy, healthy, and ready for downstream use.
Cell Type: T cells
Sample Species and Source: Human PBMCs
Cell Separation Method: Negative Selection
Capacity: 1 billion cells
This kit comes with:
Akadeum’s BACS™ Streptavidin Microbubbles
Akadeum’s Human T Cell Antibody Cocktail
Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
5mL Eppendorf Tubes
For best results, the following supplies are recommended:
Low retention 1mL pipet tips
Centrifuge with swinging bucket rotor
Akadeum’s Human T Cell Isolation Kit leverages Akadeum’s novel Buoyancy Activated Cell Separation (BACS™) technology for sample preparation that delivers a highly enriched population of healthy, viable T cells using a protocol that is fast, easy, and gentle on cells. Ideal for isolating delicate immune cells, Akadeum’s microbubble enrichment protocol occurs directly in the sample container, eliminating the need to expose cells to harsh chemicals or harmful shear forces from magnetic gradients. The microbubbles are exceptionally gentle and maintain cell health and physiology. T Cell Isolation takes about 30 minutes without the need for additional equipment or expensive consumables like magnets or columns. The end result is a high-purity population of human T cells that are happy, healthy, and ready for downstream use.
Elevated Solutions for Sample Preparation:
Exceptionally gentle T cell enrichment for happy cells
30-minute workflow is fast and easy to complete
No magnet, no column, no extra equipment
Maintain delicate T cell health and physiology
Elegant in Simplicity. Powerful in Application.
Akadeum’s microbubble technology harnesses the power of buoyancy in a fast, easy and gentle workflow without the need for additional equipment. This unique, gentle approach maintains cell health and physiology.
No Magnet. No Column. Happy Cells.
We’ve taken technology that traditionally requires additional equipment to accomplish targeted cell separation and put it into a single container where it’s self-separating. No harmful shear forces, no exposure to external forces like magnetic gradients. Stop losing your precious cells to harsh, outdated processing techniques.
Experience the benefits of a microbubble workflow. Akadeum’s Microbubbles Are:
Magnet-free, protecting your cells from harsh magnetic forces and eliminating the need for additional (and expensive!) equipment
Column-free, using microbubbles directly in the sample vial to separate cells allows for quick and easy isolation of any sample, any volume, anywhere
Gentle on cells, harnessing the targeted efficiency of affinity molecules coupled with the power of buoyancy to gently separate via flotation
Reliable and consistent, delivering fast and easy results that can be scaled for larger volumes with workflows that are compatible with nearly any downstream processing
Looking for Answers?
At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and are dedicated to helping the researchers we work with see exceptional results. If you’re interested in leveraging the microbubble platform to overcome existing hurdles in your sample preparation processes, we want to hear from you! You can speak directly to our team of scientists who are available to help you with an optimized workflow that takes advantage of our microbubble technology and solves long-standing headaches in sample preparation. Schedule a call today, we’d love to start a conversation!
Isolation of human T cells from PBMCs was performed using Akadeum Life Sciences Human T Cell Isolation Kit (#13210-120). Cells were labeled with Streptavidin-FITC and CD3-PE. Dead cells and debris were excluded from analysis.