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Human T Cell Isolation Kit

$276.50$553.00

  • Akadeum’s Human T Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles and biotinylated antibodies to enrich T cells from peripheral blood mononuclear cells
  • Negative selection enrichment method
  • With the Full Kit, process 10 samples, up to 100 million cells per sample
  • With the Trial Kit, process 3 samples, up to 100 million cells per sample
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SKU: 13210-120 Category:

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Product Details

Overview

  • Akadeum’s Human T Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles to negatively select T cells from peripheral blood mononuclear cells
  • Non-T cells (unwanted cells) are labeled with biotinylated antibodies and subsequently mixed with microbubbles
  • Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet
  • High purity human T cells are left untouched and ready for downstream applications

Advantages:

  • No column
  • No magnet
  • Up to 95% purity
  • High yield
  • Easy to use
  • Fast
  • Untouched and viable cells

Capacity: Full Kit = 1 billion cells; Trial Kit = 300 million cells

Components of Human T Cell Isolation Kit (Full Kit #13210-120) (Trial Kit #23210-120):

  • BACS Streptavidin Microbubbles
  • Human T Cell Antibody Cocktail (containing biotin-CD14, CD16, CD19, CD36, CD56, CD123, CD235ab)
  • Separation buffer

Format:

  • Antibody cocktail: Phosphate buffered solution containing 0.09% sodium azide
  • Microbubbles: Aqueous solution
  • Separation buffer: Phosphate buffered solution containing 0.5% BSA and 2 mm EDTA

Storage: Store all components at 4°C

Cell Type: T cells

Sample Species and Source: Human PBMCs

Cell Separation Method: Negative selection

Area of Research: Immunology

Data

Microbubbles were used to enrich human T cells to a purity of 94.5% from a 52.4% starting purity (shown below).

human t cell enrichment flow data

Resources

Human T Cell Isolation Kit Product Info Sheet