fbpx
Products

Mouse B Cell Isolation Kit

$320.00$640.00

  • Akadeum’s Mouse B Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles and biotinylated antibodies to enrich B cells from splenocytes
  • Negative selection enrichment method
  • With the Full Kit, process 10 samples, up to 100 million cells per sample
  • With the Trial Kit, process 3 samples, up to 100 million cells per sample
Clear
SKU: 12210-110 Category:

for more information and current discounts

Product Details

Overview

  • Akadeum’s Mouse B Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles to negatively select B cells from splenocytes
  • Non-B cells (unwanted cells) are labeled with biotinylated antibodies and subsequently mixed with microbubbles
  • Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet
  • High purity mouse B cells are left untouched and ready for downstream applications

Advantages

  • No column
  • No magnet
  • Up to 95% purity
  • High yield
  • Easy to use
  • Fast
  • Untouched and viable cells

Capacity: Full Kit = 1 billion cells; Trial Kit = 300 million cells

Components of Mouse B Cell Isolation Kit (Full Kit #12210-110) (Trial Kit #22210-110):

  • BACS Streptavidin Microbubbles
  • Mouse B Cell Antibody Cocktail (containing biotin-CD3, CD4, CD8a, CD11b, CD11c, CD49b, Gr-1, and TER119)
  • Separation buffer

Format:

  • Antibody cocktail: Phosphate buffered solution containing 0.09% sodium azide
  • Microbubbles: Aqueous solution
  • Separation buffer: Phosphate buffered solution containing 0.5% BSA and 2 mM EDTA

Storage: Store all components at 4°C

Cell Type: Naive/resting B cells

Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged. Other mouse starting materials have not been evaluated by Akadeum and may require additional user optimization

Cell Separation Method: Negative Selection

Area of Research: Immunology

Kit Type: B Cell Isolation KitRemove product image

Data

Microbubbles were used to enrich mouse splenocytes to a purity of 93.9% from a 48.7% starting purity (shown below).

mouse b cell enrichment flow data

Resources

Mouse B Cell Isolation Kit Product Info Sheet