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Mouse CD4+ T Cell Isolation Kit

$320.00$640.00

SKU: 12210-130 Categories: ,
  • Akadeum’s Mouse CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles and biotinylated antibodies to enrich CD4+ T cells from splenocytes
  • Negative selection enrichment method
  • With the Full Kit, process 10 samples, up to 100 million cells per sample
  • With the Trial Kit, process 3 samples, up to 100 million cells per sample
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Product Details

Overview

  • Akadeum’s Mouse CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles to negatively select CD4+ T cells from splenocytes
  • Non-CD4+ T cells (unwanted cells) are labeled with biotinylated antibodies and subsequently mixed with microbubbles
  • Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet
  • High purity mouse CD4+ T cells are left untouched and ready for downstream applications

Advantages:

  • No column
  • No magnet
  • Up to 96% purity
  • High yield
  • Easy to use
  • Fast
  • Untouched and viable cells

Capacity: Full Kit = 1 billion cells; Trial Kit = 300 million cells

Components of Mouse CD4+ T Cell Isolation Kit (Full Kit #12210-130) (Trial Kit #22210-130):

  • BACS Streptavidin Microbubbles
  • Mouse CD4+ T Cell Antibody Cocktail (containing biotin-B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD49b, Gr1, TCR-γδ, and TER119)
  • Separation buffer

Format:

  • Antibody cocktail: Phosphate buffered solution containing 0.09% sodium azide
  • Microbubbles: Aqueous solution
  • Separation buffer: Phosphate buffered solution containing 0.5% BSA and 2 mm EDTA

Storage: Store all components at 4°C

Cell Type: Naive / resting CD4+ T cells

Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged. Other mouse starting materials have not been evaluated by Akadeum and may require additional user optimization

Cell Separation Method: Negative selection

Area of Research: Immunology

Kit Type: T Cell Isolation Kit, CD4 T Cell Isolation Kit

Additional information

Cell Type

Resources

Mouse CD4+ T Cell Isolation Kit Product Info Sheet