Product Details
Overview
- Akadeum’s Mouse CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles to negatively select CD4+ T cells from splenocytes
- Non-CD4+ T cells (unwanted cells) are labeled with biotinylated antibodies and subsequently mixed with microbubbles
- Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet
- High purity mouse CD4+ T cells are left untouched and ready for downstream applications
Advantages:
- No column
- No magnet
- Up to 96% purity
- High yield
- Easy to use
- Fast
- Untouched and viable cells
Capacity: Full Kit = 1 billion cells; Trial Kit = 300 million cells
Components of Mouse CD4+ T Cell Isolation Kit (Full Kit #12210-130) (Trial Kit #22210-130):
- BACS™ Streptavidin Microbubbles
- Mouse CD4+ T Cell Antibody Cocktail (containing biotin-B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD49b, Gr1, TCR-γδ, and TER119)
- Separation buffer
Format:
- Antibody cocktail: Phosphate buffered solution containing 0.09% sodium azide
- Microbubbles: Aqueous solution
- Separation buffer: Phosphate buffered solution containing 0.5% BSA and 2 mm EDTA
Storage: Store all components at 4°C
Cell Type: Naive / resting CD4+ T cells
Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged. Other mouse starting materials have not been evaluated by Akadeum and may require additional user optimization
Cell Separation Method: Negative selection
Area of Research: Immunology
Kit Type: T Cell Isolation Kit, CD4 T Cell Isolation Kit
