Products

Mouse CD4+ T Cell Isolation Kit

$590.00

  • Akadeum’s Mouse CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles and biotinylated antibodies to enrich CD4+ T cells from splenocytes.
  • Negative selection enrichment method
  • Process 10 samples, up to 100  million cells per sample
SKU: 10021 Category:

Product Details

Overview

  • Akadeum’s Mouse CD4+ T Cell Isolation Kit uses streptavidin-conjugated BACS microbubbles to negatively select CD4+ T cells from splenocytes.
  • Non-CD4+ T cells (unwanted cells) are labeled with biotinylated antibodies and subsequently mixed with microbubbles.  
  • Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet.
  • High purity mouse CD4+ T cells are left untouched and ready for downstream applications.

Advantages:

  • No Column
  • No Magnet
  • Up to 96% purity
  • High Yield
  • Easy to use
  • Fast
  • Untouched and viable cells

Capacity: Kit for 109 total cells

Components of Mouse CD4+ T Cell Isolation Kit (Product # 10021):

  • BACS Streptavidin Microbubbles
  • Mouse CD4+ T Cell Antibody Cocktail (containing biotin-B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD49b, Gr1, TCR-γδ, and TER119)
  • Separation Buffer

Format:

  • Antibody cocktail: Phosphate buffered solution containing 0.09% sodium azide
  • Microbubbles: Aqueous solution
  • Separation buffer: Phosphate buffered solution containing 0.5% BSA and 2 mM EDTA
  • Storage: Store all components at 4°C

Cell Type: Naive / resting CD4+ T cells

Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged. Other mouse starting materials have not been evaluated by Akadeum and may require additional user optimization.

Cell Separation Method: Negative Selection

Area of Research: Immunology

Additional information

Cell Type

T Cell Products

Resources

Mouse CD4+ T Cell Isolation Kit Product Info Sheet