The Human CD14+ Monocyte Isolation Kit delivers a high purity population of classical human monocytes that are ready for downstream use. The BACS™ Microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of monocytes during enrichment.
Mechanism of Action: The Human CD14+ Monocyte Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich CD14+ monocytes. Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD3, CD5, CD15, CD16, CD19, CD20, CD56, CD57, CD66b, CD123, CD235ab, CD314.
How it Works: The biotinylated antibody cocktail is added to the sample to label unwanted cells. BACS™ Microbubbles are added to the sample and bind to the antibody-labeled cells. Microbubbles bound to the undesired cells, quickly and gently float the undesired cells to the surface. CD14+ monocytes, the cells of interest, are pelleted through centrifugation. Microbubble-bound cells and supernatant are easily aspirated away, leaving untouched and enriched CD14+ monocytes healthy and ready for downstream use.
Cell Type: Human CD14+ Monocytes
Sample Species and Source: Human MNCs
Cell Separation Method: Negative Selection
Capacity: 1 billion MNCs
This kit comes with:
BACS™ Streptavidin Microbubbles
Human CD14+ Monocyte Biotin Antibody Cocktail
Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
5 mL Tubes
For best results, the following supplies are recommended:
20 rpm tube rotator for mixing
Centrifuge with swinging bucket rotor
30 μm cell strainer
The Human Monocyte Isolation Kit leverages Akadeum’s novel Buoyancy Activated Cell Sorting (BACS™) technology to deliver a highly enriched population of healthy, viable CD14+ monocytes. BACS™ Microbubbles are fast, easy-to-use, and gentle on cells. The BACS™ Microbubble isolation platform minimizes handling by enabling single-tube processing, limiting unnecessarily loss of cells. The monocyte isolation protocol is fast and simple, taking just 30 minutes and not requiring magnets or columns. The result is a population of highly pure classical monocytes that are healthy and ready for downstream use.
Elevated Solutions for Sample Preparation
Exceptionally gentle CD14+ monocyte enrichment
Fast and easy workflow; complete in ~30 minutes
Single tube processing that minimizes sample handling and loss of target cells
Elegant in Simplicity, Powerful in Application
BACS™ Microbubble technology harnesses the power of buoyancy to provide a fast, easy, and gentle cell isolation. Our novel technology couples performance, ease, and quality, bringing cell separation to the next level.
Experience the benefits of a Microbubble workflow. Akadeum’s Microbubbles are:
Magnet-free: Cells are not subjected to harsh magnetic forces.
Column-free: BACS™ workflow is easy-to-use and does not require onerous and expensive columns.
Gentle on cells: Harnessing the power of buoyancy, BACS™ Microbubbles swiftly and gently remove unwanted cells via flotation.
Fast, reliable, and scalable: The simple BACS™ Microbubbles protocol delivers reproducible results and can be easily scaled for larger volumes.
Looking for Answers?
At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and dedicate ourselves to helping researchers obtain exceptional results. If you’re interested in leveraging our BACS™ Microbubble platform to overcome existing hurdles in your cell isolation workflow, we want to hear from you! You can speak directly with an Akadeum scientist available to help you with an optimized workflow. Schedule a call today; we’d love to start a conversation!
Untouched CD14+ monocytes were isolated from MNCs using the Human CD14+ Monocyte Isolation Kit (13210-220). Isolated cells were labeled with CD14-PE and CD16-APC/Fire750. The fluorescently labeled cells were analyzed by flow cytometry. Debris and dead cells were excluded from analysis.