Resources
App Notes
Unmatched Scalability, from Process Development to Manufacturing
As your cell therapy workflow scales, Akadeum's technology scales with you! From 50 million cell experiments to tens of billions, viability is maintained while time decreases.
High Purity T Cells and Reduced Key Impurities
A well-known CDMO achieves high purity T Cells while reducing key cellular impurities, improving their transduction efficiency and cell therapy workflow.
In this app note, we demonstrate the effectiveness and efficiency of the microbubble workflow for removing residual RBC contamination from human PBMC samples. Download the app note and learn more.
Download this app note to understand how to successfully isolate cells of low abundance, while saving time and maintaining high viability, through a combination of Akadeum’s microbubble based CD4+ T cell enrichment and subsequent Treg isolation using NanoCellect’s WOLF® Cell Sorter.
We have expanded our microbubble platform by functionalizing our revolutionary buoyancy-activated separation (BACS™) technology for RNA isolation in saliva samples, and we’re optimizing a workflow that allows for quick and easy RNA extraction in larger volume samples.
Ultimate Guide to Microbubbles
The isolation of highly purified cells has become a necessity for life science research. Cell isolation is utilized in everything from the simple removal of red blood cells from samples to CAR T-cell therapy.
Blog
Minimize Sample Manipulation to Protect Fragile Cells
When working with fragile cell types—like stem cells, regulatory T cells, or other rare populations—every extra...
GMP Cell Therapy Kits Deliver Translation-Readiness
The rapid rise of cell therapies is transforming how we treat disease. However, every step from discovery to...
Unmatched Flexibility: Manual T Cell Isolation with Akadeum’s Microbubble Kits
Not every cell therapy lab can access high-throughput automation, but that shouldn’t mean sacrificing quality,...
Streamlining Cell Therapy Workflows: Integrating Microbubbles into the Cocoon® Platform
Imagine unlocking greater flexibility in your cell therapy workflow while preserving cell health, improving yield,...
The Evolution of CLL Treatment and the Role of CAR T Cell Therapy
Chronic Lymphocytic Leukemia (CLL) is a complex blood cancer that affects the immune system by disrupting normal...
Transforming Multiple Myeloma Treatment With CAR T Cell Therapy
Multiple Myeloma (MM) is a cancer of plasma cells that disrupts normal immune function and blood cell production....
Diffuse large B cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin’s lymphoma (NHL), accounting for...
Overcoming B-ALL With Enhanced CAR T Manufacturing
B cell acute lymphoblastic leukemia (B-ALL) is an aggressive cancer affecting the bone marrow and blood. When...
From Beads to Microbubbles: T Cell Isolation
Whether for basic science or advanced applications like CAR T therapy, the efficiency and precision of cell...
Negative Selection at Scale: Driving the Next Era of CAR T Immunotherapy Manufacturing
Rising cancer rates have been featured heavily in the news this year, especially among younger adults. As cancer...
Case Studies
Cell Separation Made Simple: Microbubbles Deliver Consistency and Flexibility for Your Workflow
Akadeum’s microbubble kits are built with universal compatibility and repeatable top performance in mind. Whether...
Poseida Therapeutics, a clinical-stage allogeneic-focused therapeutic developer leveraging proprietary technologies...
Comprehensive Cell Solutions (CCS), a business unit of the New York Blood Center Enterprises, is a Contract...
Akadeum’s AlerionTMMicrobubble Cell Separation System: A Case Study in T Cell Banking
Akadeum Life Sciences has partnered with a cell therapy AlerionTM Microbubble Cell Separation System beta customer...
The Alerion™, in combination with the BioRise™ consumable and microbubble technology, provides the first...
Enriching CD3+ T Cells with Alerion™ Microbubble Cell Separation System
A cell therapy company sought to improve its CD3+ T cell isolation process. To address the challenges it faces with...
Using Akadeum’s Human CD4+ T Cell Isolation to Study Pathogenesis of Human Hepatotropic Viruses
Use of Akadeum’s gentle and effective Human CD4+ T Cell Isolation Kit results in a highly enriched sample of...
Use of Akadeum’s gentle Mouse B Cell Isolation kit results in a highly enriched sample of B cells that are...
“The resulting sample looked perfect after using microbubbles – very happy with the product.” Get an inside...
Using Akadeum’s Mouse B Cell Isolation Kit to study B cell differentiation into plasma cells
Akadeum’s gentle Mouse B Cell Isolation kit produces high purity B cell population without affecting physiology...
Events
The CAR-TCR Summit is the world’s leading conference dedicated to the end-to-end coverage of every stage of cell...
Akadeum is honored to be selected to moderate a panel on a critical topic—quality, speed, and cost—in addition...
Akadeum to Co-Sponsor CGT Symposium in San Diego
Akadeum is proud to be a sponsor for the upcoming Hope Is Not a Workflow: Addressing the Hard Truths of CGT...
Meet Akadeum at BIO International Convention 2025
Meet Akadeum at BIO International Convention 2025, Boston, June 16–19, Boston Convention and Exhibition Center....
Akadeum Highlights Integration at ASGCT Alongside Leading Cell Therapy Partners
Akadeum is participating alongside partners at the forefront of the cell therapy space in the American Society of...
Akadeum to Present at Biotech Showcase 2025 in San Francisco
Innovation takes center stage on January 13th, 2025, as Dr. Brandon McNaughton, CEO of Akadeum Life Sciences,...
Akadeum at Advanced Therapies Week 2025 in Dallas
Akadeum Announces Alerion™ Microbubble Cell Separation System Availability After a year of testing onsite with...
Comprehensive Cell Solutions and Akadeum at Advanced Therapies Congress 2024
Join us at the Advanced Therapies Congress, November 12 -13, 2024, held at the Pennsylvania Convention Center in...
Join the Akadeum team in Phoenix on October 7- 9, 2024, at the annual Cell and Gene Meeting on the Mesa held at the...
Akadeum’s Brandon McNaughton to Present at Biotech Showcase 2024
Join the Akadeum team in San Francisco on January 8-10, 2024 at the annual Biotech Showcase event. Recognized...
FAQs
What is buoyancy-activated cell sorting (BACS)?
BACS is Akadeum’s proprietary cell separation technology that uses buoyant microbubbles to isolate specific cell populations utilizing only the power…
What are microbubbles?
Akadeum’s microbubbles are hollow silica spheres that are on average 12 microns.
How big are microbubbles?
Microbubbles average 12 um in diameter. Learn more about microbubbles technology.
What is the Separation Buffer?
Ca2+, Mg2+ free Dulbecco’s PBS containing 2mM EDTA, and 0.5% bovine serum albumin (BSA). BSA must be biotin free to…
After sitting in the refrigerator, all the microbubbles have separated out of solution. Is there something wrong?
No, this is normal due to the buoyant properties of microbubbles. The solution will need to be mixed well before…
I don’t have an aspirator/vacuum available in my lab, can I still use Akadeum microbubbles?
Aspiration is the best and easiest way to remove the microbubble layer after centrifugation. Pipetting off the bubble layer is…
What are the minimum and maximum cell numbers that can be processed with Akadeum’s kits?
It is not recommended to process under 10 million cells per sample. The maximum number of cells processed is dependent…
What selection method is used for Akadeum’s kits?
Akadeum’s Human T Cell Leukopak Isolation Kit is the first ever negative selection kit that can meet the scale requirements…
What is meant by washed leukopak material?
Washed leukopak material refers to a leukopak that has been processed to reduce platelets, exchange buffer, and concentrate the cells.…
Can unwashed leukopaks be used with Akadeum’s kits?
Yes, Akadeum has successfully processed raw leukopaks. Please email techsupport@akadeum.com to learn more.
Can Akadeum’s kits be used with cryopreserved samples?
Yes, Akadeum’s kits are designed to work with both fresh and cryopreserved samples.
Are Akadeum’s kits for autologous or allogeneic workflows?
Akadeum’s kits can be integrated into both autologous and allogeneic workflows.
How many cells can be processed in a single run?
The number of cells processed depends on the specific kit or customized protocol. In general, each BioRise™ consumable can process…
How many donors can be processed on an Alerion™ per shift?
Two leukopaks can be processed simultaneously, each in their BioRise™ consumable which provides a closed system. Processing two consumables at…
Does Akadeum have a demo program for the Alerion™?
Yes, reach out to your Account Executive or email info@akadeum.com to learn more!
Can I do back-to-back isolation to improve purity?
Yes. This can be specifically helpful when using the Dead Cell Removal Microbubble Kit or the Human Red Blood Cell…
Which cell types can be processed using Akadeum’s Dead Cell Removal Microbubble Kit?
Akadeum’s Dead Cell Removal Microbubble Kit utilizes Annexin V-coated microbubbles to selectively bind phosphatidylserine, a marker exposed on the surface…
Can the Dead Cell Removal Microbubble Kit be used if the sample is under 35% viability?
Although the initial cell viability is below the recommended threshold, successful outcomes have been achieved by performing the protocol twice…
News
Akadeum Life Sciences, the pioneer in gentle buoyant cell separation technology, and BioIVT, a leading global...
Akadeum Life Sciences, a leader in buoyancy-based cell separation technology, will present significant milestone...
Cayman Chemical, a global provider of life science research tools, and Akadeum Life Sciences, a leader in...
Akadeum’s History of Introducing Innovative New Products at ASGCT Continues
Akadeum, the sole proprietor of ground-breaking microbubble technology, is expanding its market-leading product...
Brandon McNaughton’s Interview Featured on PharmTech and BioPharm International
Brandon McNaughton, Founder and CEO of Akadeum Life Sciences, recently conducted an interview with Grant Playter,...
Cell Isolation Technology Demonstration at We Make Health Fest
We demonstrated Akadeum’s cell isolation and sorting technology at the We Make Health Fest on August 16,...
CEO Brandon McNaughton Publishes Cell Therapy Cancer Treatments Opinion Piece
Akadeum Life Sciences CEO and Co-Founder Brandon McNaughton’s article “Opinion: Cell Therapy Cancer Treatments...
In a Head-to-Head Comparison Akadeum’s Mouse B Cell Isolation Microbubbles Generated 32% More Positive...
EDRN 2021 Poster Presentation: Enrichment of Rare Circulating Cells Using Microbubbles
Akadeum recently had the opportunity to present a poster presentation at The 12th Scientific Workshop of the Early...
New Poster from Akadeum, in collaboration with Albert Einstein College of Medicine: Enhanced Enrichment of iNKT...
Posters
All-in-One CAR-T Manufacturing Using Next-Generation Technology
In this poster presented by Limula at ESGCT 2025, learn how Limula is leveraging Akadeum’s microbubbles in an...
Microbubbles are an effective solution to cell separation challenges in cell therapy. This data demonstrates the...
Healthier Cells with Akadeum’s Microbubbles: A Novel Approach to Gentle and Efficient Cell Isolation
Akadeum’s microbubbles offer a simple yet powerful shift in cell separation – negative selection that...
Evaluating Buoyancy Activated Cell Sorting for T Cell Isolation in CAR-T Manufacturing
As the CAR T and TCR landscape evolves, robust manufacturing solutions that enable increased access to these...
End-to-End Production of T Cells on the Cocoon Platform, using BACS for Direct Isolation
In this work we present an automated 9-day T cell isolation and manufacturing process on the...
Videos
Discover how Akadeum’s microbubble technology works in just four simple steps that deliver healthier, more potent...
Improving Cell Separation for Better Therapies and Greater Access
Akadeum CEO Brandon McNaughton returns to Cell & Gene’s Meeting on the Mesa to present significant regulatory...
Akadeum’s buoyancy-based cell separation allows for an unique in-bag negative selection protocol that can be...
Cells are isolated in-bag then transferred from the bag to any Wilson Wolf G-Rex® bioreactor. Cells are then...
In-Bag Cell Separation and Wilson Wolf G-Rex® Bioreactor Compatibility
In this video, Akadeum Life Sciences Sr. Field Application Scientist Crisha Christensen demonstrates how to combine...
In-Bag Cell Separation with Thermo Fisher Rotea® Compatibility
In this video, Akadeum Life Sciences’ Senior Field Application Scientist Crisha Christensen demonstrates how to...
In this step-by-step video, Akadeum Life Sciences Sr. Field Application Scientist Crisha Christensen demonstrates...
The Alerion™ Microbubble Cell Separation System: for Healthier Cells
Take a deeper dive into the Alerion™ and the BioRise™ consumable with our Senior Director of R&D, Dr. Bill...
Alerion™ Microbubble Cell Separation System – Scalable. Fast. Efficient.
Explore the components of the Alerion™ Microbubble Cell Separation System in this minute and a half video, and...
Human T Cell Activation and Expansion Kit Protocol
Our Human T Cell Activation and Expansion Kit is now available for research use. This video will walk you through...