Resources
Akadeum was established to fundamentally change the way that isolating cells and other biological targets is approached. Continue reading to find out more about our methods and products.
Improving Cell Separation for Single Cell RNA Sequencing
RNA-Sequencing Medical advancements have provided researchers with strategies that change the way in which we understand biology....
What is Tumor-Infiltrating Lymphocyte (TIL) Therapy?
Immunotherapy Immunotherapy is the practice of manipulating T cells to treat cancer. Also called adoptive cell therapies, there...
Cell Therapies Medical research and cancer treatment strategies are constantly adapting to overcome new challenges. When more...
CAR T Cells: How These Cancer Fighting T Cells are Harvested and Manufactured for Immunotherapy
Immunotherapy Immunology is the study of the immune system and what it can do, immunotherapy is the study of how to do things the...
What is Protein Therapy and What Role Does Cell Separation Play in it?
Proteins are large molecules in the body that play a critical role in virtually everything that happens. Movement, maturation, and...
Cost Comparison of Cell Separation Methods
Cell Separation Immunology is an evolving field of study that constantly requires new equipment and methods to keep up with...
Cell Death and Sample Viability
Cell Loss When performing research, it’s easy to forget that individual cells are living things. Each one has its own role and...
Adoptive Cell Therapy in Cancer Treatment
Adoptive Immunotherapy The human body is naturally equipped with tools to fight off infectious diseases and harmful pathogens. An...
DATA BRIEF: Expanding COVID-19 Testing
Early Data from Akadeum’s RNA Extraction Project for Pooled Saliva Samples Current COVID-19 saliva testing is...
What Receptors Do T Cells Have?
T Cell Receptors T Cell Receptors (TCRs) are protein complexes found on the surface of T cells or T lymphocytes responsible for...
Improving Cell Separation for Single Cell RNA Sequencing
RNA-Sequencing Medical advancements have provided researchers with strategies that change the way in which we understand biology....
What is Tumor-Infiltrating Lymphocyte (TIL) Therapy?
Immunotherapy Immunotherapy is the practice of manipulating T cells to treat cancer. Also called adoptive cell therapies, there...
Cell Therapies Medical research and cancer treatment strategies are constantly adapting to overcome new challenges. When more...
CAR T Cells: How These Cancer Fighting T Cells are Harvested and Manufactured for Immunotherapy
Immunotherapy Immunology is the study of the immune system and what it can do, immunotherapy is the study of how to do things the...
What is Protein Therapy and What Role Does Cell Separation Play in it?
Proteins are large molecules in the body that play a critical role in virtually everything that happens. Movement, maturation, and...
Cost Comparison of Cell Separation Methods
Cell Separation Immunology is an evolving field of study that constantly requires new equipment and methods to keep up with...
Cell Death and Sample Viability
Cell Loss When performing research, it’s easy to forget that individual cells are living things. Each one has its own role and...
Adoptive Cell Therapy in Cancer Treatment
Adoptive Immunotherapy The human body is naturally equipped with tools to fight off infectious diseases and harmful pathogens. An...
DATA BRIEF: Expanding COVID-19 Testing
Early Data from Akadeum’s RNA Extraction Project for Pooled Saliva Samples Current COVID-19 saliva testing is...
What Receptors Do T Cells Have?
T Cell Receptors T Cell Receptors (TCRs) are protein complexes found on the surface of T cells or T lymphocytes responsible for...
Akadeum is a semi-finalist in the XPRIZE Rapid Covid Testing competition.
Akadeum is pleased to announce that we are a semi-finalist in the XPRIZE Rapid Covid Testing competition. We have recently...
Akadeum & NanoCellect Collaborating to Improve Rare-Cell Workflows and Results
Akadeum Life Sciences, Inc., creators of a next generation floatation-based isolation platform, and NanoCellect Biomedical, Inc.,...
Akadeum Announces First Closing of Series A Financing Round
Akadeum closes funding to further commercialize its BACS™ Microbubble platform for next-generation sample preparation...
Akadeum and Agilent Team Up to Explore New Testing Workflow
Novel Akadeum microbubble technology can be the answer to improved quality and reduced costs across multiple fields of...
Akadeum Launches Seven New Products at Immunology 2019 Conference in San Diego
Ann Arbor-based biotech company, Akadeum Life Sciences, announced the release of seven new products based on its proprietary...
George Dunbar Joins Akadeum’s Board of Directors
Akadeum Life Sciences has recently added George Dunbar to our Board of Directors. George has spent his career as an executive...
First-of-Their-Kind Microbubble Products Commercially Available
We are excited to announce that we making our microbubble-based T cell and B cell isolation kits available for purchase to early...
Akadeum Advances Next Generation Cell Isolation Technology
We have successfully demonstrated use of microbubbles for isolation of human T-cells and mouse B-cells. As a result, we are...
Akadeum Recognized for Best New Technology at CYTO Innovation
Akadeum’s groundbreaking microbubble technology—which allows for fast, easy removal of target cells from biological...
Microbubbles Float Through Their First Flow Cytometry Conference
Akadeum recently attended the GLIIFCA 2016 meeting. John Younger, our CTO, gave a great talk to introduce Akadeum’s cell...
How big are microbubbles?
In particular, we currently use polydisperse microbubbles that are 16–18 um in diameter. Read this blog to learn more.
What are microbubbles made of?
Akadeum’s microbubbles are made of thin shells of glass with a gaseous core. Read this blog to learn more.
How many cells can a microbubble lift?
When isolating fully dispersed cells (such as in a Ficoll or leukopheresis PBMC prep), we have seen microbubbles carrying as many as 4 cells. Cells…
What is the Separation Buffer?
Ca2+, Mg2+ free Dulbecco’s PBS containing 2mM ETDA, 0.09% sodium azide, and 0.5% bovine serum albumin (BSA). BSA must be biotin free as to not…
Does Akadeum have a recommendation for biotin free BSA?
Millipore Sigma Bovine Serum Albumin – Product #A7030
Can Akadeum microbubbles be analyzed by flow cytometry?
Yes. Analyzing samples of microbubbles or microbubbles bound to cells can be performed but high concentrations of microbubbles have been found to clog the SIP…
My spleens are very large, is it possible to process samples containing >100 million cells in a single separation?
No. Samples in excess of 100 million cells should be divided and multiple separations performed. The cells can be recombined afterward. This is a result…
What if my samples are small (<10 million cells)?
When processing samples that are less than 10 million white cells/splenocytes, Akadeum recommends the following protocol changes: Replacing the provided 5 mL centrifuge tubes with…
Are Akadeum microbubble enriched samples compatible with flow cytometry?
Yes. Residual microbubbles in samples will not interfere with flow cytometry. Microbubbles are larger than many cell types and have very bright side scatter so…
Can I culture the isolated cells after isolation with Akadeum microbubbles?
Yes. After removal of the microbubble layer, the remaining cell pellet can be resuspended in any desired buffer and cultured or analyzed with other methods.
I am using an Akadeum mouse kit, do I have to lyse the red cells?
Lysing red cells is recommended to increase purity. The antibody cocktails contain a TER119 antibody to capture and remove red cells. However, depending on the…
Can I do back to back isolations to improve purity?
Yes, but any improvement in purity will be accompanied by additional loss of desired cells.
Why is mixing volume 50% of the total volume?
This ensures adequate opportunity for the microbubbles to find lower abundance cell types that are targeted. Using a higher percentage (ex. 70%) will perform similarly,…
I’m having trouble aspirating the microbubbles, what can I do?
Please watch the video demonstrating aspiration of microbubbles at www.Akadeum.com. Working with microbubbles can take a bit of practice.
What if there are a lot of microbubbles remaining (stuck to the sides of the tube) after aspiration? Is this a problem?
As these microbubbles should only contain cells that are targeted for removal, they should not cause any issues. However, if you wish to remove them,…
Can I decrease the biotin antibody incubation time?
No. Lower incubation times at 4°C will result in lower purity.
Can I perform the biotin antibody incubation at room temperature for a shorter time?
Akadeum has not determined if shorter room temperature incubations will change product performance so it is not recommended at this time.
After sitting in the refrigerator, all the microbubbles have separated out of solution. Is there something wrong?
This is normal. The solution will need to be mixed well before every use.
How do I know if the microbubbles are mixed enough before using them?
The microbubbles should be a homogenous white solution. Rocking the vial, a brief vortex, or mixing with a pipet can all help. After mixing, check…
How fast is too fast for mixing the microbubbles?
Trituration should be performed at approximately 0.5 to 1 stroke per second. See the video at www.Akadeum.com for a demonstration.
I’m worried about trituration impacting my cells, is there an alternative mixing method?
Akadeum has found slow mixing by trituration to be the most effective and quickest method for mixing microbubbles with cells.
I don’t have low retention pipet tips; can I use standard tips?
Low retention tips are not required but more microbubbles will adhere to standard tips. This may impact isolation performance.
I don’t have access to a swinging bucket rotor centrifuge, can I still use Akadeum microbubbles?
Microbubbles will still rise to the top in a fixed rotor centrifuge however the microbubble layer will not be even. This can make aspirating more…
I don’t have an aspirator/vacuum available in my lab, can I still use Akadeum microbubbles?
Aspiration is the best/easiest way to remove the microbubble layer after centrifugation. Pipetting off the bubble layer is not very effective and pipetting the cell…
How are Akadeum kits shipped?
Kits are shipped on ice packs with overnight delivery.
How should I store components?
Separation Buffer, microbubbles and antibody cocktail should be stored at 4 degrees Celsius.
“It was super quick - just 2 or 3 steps. Akadeum was quicker than any other kit I have used before.”
“Akadeum enrichment kit worked absurdly better than [magnetic bead separation].”
“It was super quick - just 2 or 3 steps. Akadeum was quicker than any other kit I have used before.”
“Akadeum enrichment kit worked absurdly better than [magnetic bead separation].”