Resources
Akadeum was established to fundamentally change the way that isolating cells and other biological targets is approached. Continue reading to find out more about our methods and products.
Types of Cell Separation: Differences Between Extraction and Applications for Separation Assays
Types of Cell Separation: Differences Between Extraction and Applications for Separation Assays Cell separation is an integral...
Human B Cell Isolation: Protocol and Purpose of Human B Cell Isolation From Blood
Human B Cell Isolation: Protocol and Purpose B cells and T cells share a common goal: to protect the human body from harmful...
Self-Antigens vs. Non-Self Antigens: Examples & Cluster of Differentiation Markers
Antigens are molecules present on the surface of cells that bind to receptors on antibodies or on the surface of lymphocytes....
Achieve Gentle Mouse Naïve Cell Isolation Using Akadeum’s Microbubble Naïve Mouse Kits
In working with biological samples for life science research, sample preparation is a critical first step to isolate the specific...
Human RBC Depletion vs Mouse RBC Depletion: Differences in Protocols & Applications
Sample preparation is often a critical first step in any number of life science workflows. Due to the inherent complexity within...
High Throughput Human T Cell Enrichment for Immunological Research
High Throughput Human T Cell Enrichment The field of immunological research is continuously expanding. More and more things are...
Gentle Mouse B Cell Enrichment: Strategies for Preserving Fragile Mouse B Cells
Mouse B Cell Enrichment: How to Culture Isolated Mouse B Cells The primary function of B cells is antibody production. They are...
Necrosis Vs. Apoptosis: Processes, Necrotic Cell Death, & Apoptosis Steps
The stages of cell death vary depending on the way in which a cell dies. When discussing cell death, there are two main types:...
Regulatory T Cells: Function of CD4, CD25 T Cells, and FOXP3 Treg Cells
The human immune system is made up of many different cells–some of which are responsible for identifying unfamiliar pathogens,...
Use of Akadeum’s gentle and effective Human CD4+ T Cell Isolation Kit results in a highly enriched sample of human CD4+ T...
Types of Cell Separation: Differences Between Extraction and Applications for Separation Assays
Types of Cell Separation: Differences Between Extraction and Applications for Separation Assays Cell separation is an integral...
Human B Cell Isolation: Protocol and Purpose of Human B Cell Isolation From Blood
Human B Cell Isolation: Protocol and Purpose B cells and T cells share a common goal: to protect the human body from harmful...
Self-Antigens vs. Non-Self Antigens: Examples & Cluster of Differentiation Markers
Antigens are molecules present on the surface of cells that bind to receptors on antibodies or on the surface of lymphocytes....
Achieve Gentle Mouse Naïve Cell Isolation Using Akadeum’s Microbubble Naïve Mouse Kits
In working with biological samples for life science research, sample preparation is a critical first step to isolate the specific...
Human RBC Depletion vs Mouse RBC Depletion: Differences in Protocols & Applications
Sample preparation is often a critical first step in any number of life science workflows. Due to the inherent complexity within...
High Throughput Human T Cell Enrichment for Immunological Research
High Throughput Human T Cell Enrichment The field of immunological research is continuously expanding. More and more things are...
Gentle Mouse B Cell Enrichment: Strategies for Preserving Fragile Mouse B Cells
Mouse B Cell Enrichment: How to Culture Isolated Mouse B Cells The primary function of B cells is antibody production. They are...
Necrosis Vs. Apoptosis: Processes, Necrotic Cell Death, & Apoptosis Steps
The stages of cell death vary depending on the way in which a cell dies. When discussing cell death, there are two main types:...
Regulatory T Cells: Function of CD4, CD25 T Cells, and FOXP3 Treg Cells
The human immune system is made up of many different cells–some of which are responsible for identifying unfamiliar pathogens,...
Use of Akadeum’s gentle and effective Human CD4+ T Cell Isolation Kit results in a highly enriched sample of human CD4+ T...
Akadeum Life Sciences Announces New Dead Cell Removal Kit Using Microbubble Technology
This new buoyant separation method creates a more effective way for researchers to use valuable samples with a higher retention...
Akadeum’s First Rising Innovators Research Award Recipient
Akadeum is thrilled to announce the first recipient of the Rising Innovators Research Award! Congratulations to Giselle Borges...
Akadeum’s Mouse B Cell Isolation Kit Used in New Research
Akadeum's Mouse B Cell Isolation Kit was used in an article recently published in Cell Reports: Negative feedback by...
Akadeum Life Sciences, Inc. Raises $17.5M Financing Round Led by Arboretum Ventures
Akadeum Life Sciences closes an oversubscribed round and plans to expand commercialization of proprietary biological separation...
New Researcher Spotlight from Harvard: Using Akadeum’s Human CD4+ T Cell Isolation
A research group led by Professor Georg Lauer at Massachusetts General Hospital and the Harvard Medical School recently used...
New Poster from Akadeum, in collaboration with Albert Einstein College of Medicine: Enhanced Enrichment of iNKT cells from Mouse...
New Poster from Akadeum, in collaboration with Fluidigm: Multiplexed Depletion of Cells from Whole Blood Samples Using...
Akadeum to present two posters at the CYTO Virtual Conference in June
Coming in June: Join Akadeum at CYTO 2021! Akadeum has had two abstracts accepted for CYTO Virtual Interactive 2021, which is...
Poster Presentation: A Superior Dead Cell Removal Platform Using Akadeum’s BACS Microbubbles Akadeum is pleased to announce...
Akadeum recently had the opportunity to present a poster presentation at The 12th Scientific Workshop of the Early Detection...
How big are microbubbles?
In particular, we currently use polydisperse microbubbles that are 16–18 um in diameter. Learn more about microbubbles technology.
What are microbubbles made of?
Akadeum’s microbubbles are made of thin shells of glass with a gaseous core. Learn more about microbubble technology.
How many cells can a microbubble lift?
When isolating fully dispersed cells (such as in a Ficoll or leukopheresis PBMC prep), we have seen microbubbles carrying as many as 4 cells. Cells…
What is the Separation Buffer?
Ca2+, Mg2+ free Dulbecco’s PBS containing 2mM ETDA, and 0.5% bovine serum albumin (BSA). BSA must be biotin free as to not interfere with kit…
Does Akadeum have a recommendation for biotin free BSA?
Millipore Sigma Bovine Serum Albumin – Product #A7030
Can Akadeum microbubbles be analyzed by flow cytometry?
Yes. Analyzing samples of microbubbles or microbubbles bound to cells can be performed but high concentrations of microbubbles have been found to clog the SIP…
My spleens are very large, is it possible to process samples containing >100 million cells in a single separation?
No. Samples in excess of 100 million cells should be divided and multiple separations performed. The cells can be recombined afterward. This is a result…
What if my samples are small (<10 million cells)?
When processing samples that are less than 10 million white cells/splenocytes, Akadeum recommends the following protocol changes: Replacing the provided 5 mL centrifuge tubes with…
Are Akadeum microbubble enriched samples compatible with flow cytometry?
Yes. Residual microbubbles in samples will not interfere with flow cytometry. Microbubbles are larger than many cell types and have very bright side scatter so…
Can I culture the isolated cells after isolation with Akadeum microbubbles?
Yes. After removal of the microbubble layer, the remaining cell pellet can be resuspended in any desired buffer and cultured or analyzed with other methods.
I am using an Akadeum mouse kit, do I have to lyse the red cells?
Lysing red cells is recommended to increase purity. The antibody cocktails contain a TER119 antibody to capture and remove red cells. However, depending on the…
Can I do back to back isolations to improve purity?
Yes, but any improvement in purity will be accompanied by additional loss of desired cells.
Why is mixing volume 50% of the total volume?
This ensures adequate opportunity for the microbubbles to find lower abundance cell types that are targeted. Using a higher percentage (ex. 70%) will perform similarly,…
I’m having trouble aspirating the microbubbles, what can I do?
Please watch the video demonstrating the aspiration of microbubbles. Working with microbubbles can take a bit of practice.
What if there are a lot of microbubbles remaining (stuck to the sides of the tube) after aspiration? Is this a problem?
As these microbubbles should only contain cells that are targeted for removal, they should not cause any issues. However, if you wish to remove them,…
Can I decrease the biotin antibody incubation time?
No. Lower incubation times at 4°C will result in lower purity.
Can I perform the biotin antibody incubation at room temperature for a shorter time?
Akadeum has not determined if shorter room temperature incubations will change product performance so it is not recommended at this time.
After sitting in the refrigerator, all the microbubbles have separated out of solution. Is there something wrong?
This is normal. The solution will need to be mixed well before every use.
How do I know if the microbubbles are mixed enough before using them?
The microbubbles should be a homogenous white solution. Rocking the vial, a brief vortex, or mixing with a pipet can all help. After mixing, check…
How fast is too fast for mixing the microbubbles?
Trituration should be performed at approximately 0.5 to 1 stroke per second. See the video at www.Akadeum.com for a demonstration.
I don’t have low retention pipet tips; can I use standard tips?
Low retention tips are not required but more microbubbles will adhere to standard tips. This may impact isolation performance.
I don’t have access to a swinging bucket rotor centrifuge, can I still use Akadeum microbubbles?
Microbubbles will still rise to the top in a fixed rotor centrifuge however the microbubble layer will not be even. This can make aspirating more…
I don’t have an aspirator/vacuum available in my lab, can I still use Akadeum microbubbles?
Aspiration is the best/easiest way to remove the microbubble layer after centrifugation. Pipetting off the bubble layer is not very effective and pipetting the cell…
How are Akadeum kits shipped?
Cell Separation Kits are shipped on ice packs with overnight delivery.
How should I store components?
Separation Buffer, microbubbles and antibody cocktail should be stored at 4 degrees Celsius.
“Akadeum cell isolation kits isolate high purity samples in a matter of minutes, no magnets needed!”
“I like that [the microbubble workflow] is streamlined compared to magnetics…generally very easy to use. The ability to get four... ”
“The resulting sample looked perfect after using microbubbles - very happy with the product.”
“Akadeum cell isolation kits isolate high purity samples in a matter of minutes, no magnets needed!”
“I like that [the microbubble workflow] is streamlined compared to magnetics…generally very easy to use. The ability to get four... ”
“The resulting sample looked perfect after using microbubbles - very happy with the product.”