Products

Mouse Naïve T Cell Isolation Kit

$320.00$640.00

SKU: 12210-160 Category:
  • Akadeum’s Mouse Naïve T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles to negatively select naïve T cells from mouse splenocytes
  • Negative selection enrichment method
  • With the Full Kit, process 10 samples, up to 100 million cells per sample
  • With the Trial Kit, process 3 samples, up to 100 million cells per sample
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Product Details

Overview

  • Akadeum’s Mouse Naïve T Cell Isolation Kit uses streptavidin-conjugated BACS™ microbubbles to negatively select naïve T cells from mouse splenocytes.
  • Non-naïve T cells (unwanted cells) are labelled with biotinylated antibodies targeting B220 (CD45R), CD11b, CD11c, CD24, CD25, CD44, CD49b, Gr-1 and TER-119, and subsequently mixed with microbubbles.
  • Once bound to unwanted cells, microbubbles enable rapid cell removal without a column or magnet.
  • High purity mouse naïve T cells are left untouched and ready for downstream applications.

Advantages:

  • No column
  • No magnet
  • Up to 96% purity
  • High yield
  • Easy to use
  • Fast
  • Untouched and viable cells

Capacity: Full Kit = 1 billion cells; Trial Kit = 300 million cells

Components of Mouse T Cell Isolation Kit (Full Kit #12210-120) (Trial Kit #22210-120):

  • Mouse naïve T cell biotin-antibody cocktail
  • Streptavidin BACSTM microbubbles
  • Separation Buffer

Storage: Store microbubbles, antibody cocktail, and Separation Buffer at 4°C

Cell Type: Naive T cells

Sample Species and Source: Mouse spleen. Lysis of red blood cells prior to use is strongly encouraged.

Cell Separation Method: Negative selection

Area of Research: Immunology

Kit Type: T Cell Isolation Kit

Data

Example of a Mouse Naïve T Cell Isolation

Fresh murine naïve T cells were isolated from a heterogenous splenocyte population. Dead cells and debris were excluded from analysis and naïve T cells were labeled with CD62L-APC and CD3-FITC (shown below).

Resources

Mouse Naive T Cell Isolation Kit Product Info Sheet