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Dead Cell Removal Microbubble Kit

$490.00

SKU: 11510-211E Category:

The Dead Cell Removal Microbubble Kit was developed with BACS™ Microbubbles for depleting dead and dying cells from biological samples while maintaining the health and physiology of viable cells of interest.

  • This kit contains 1 mL of Dead Cell Removal Microbubbles
  • Fast & easy workflow effectively removes dead cells in 25 minutes
  • Isolated viable cells are ready for flow sorting, single-cell sequencing, pharmacologic studies, or other downstream assays

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Product Details

Spend less time preparing your sample and more time analyzing it with our 25-minute, industry-leading microbubble protocol. BACS™ Dead Cell Removal Microbubbles eliminate dead cells, enabling better science with more isolated, viable cells in a cleaner sample. 

Mechanism of Action: During apoptosis, phosphatidylserine (PS) – a phospholipid normally found on the inner surface of the plasma membrane – is redistributed to the extracellular surface. The relocation of PS to the outer surface of the cell membrane provides an effective target for differentiating dead cells from viable cells within a biological sample. The Dead Cell Removal Kit utilizes BACS™ Microbubbles coated with Annexin V, a PS binding molecule, to capture and float dead cells away.

How it Works: The Dead Cell Removal Microbubble Kit are mixed into the sample where they bind to dead and dying cells. Using their inherent buoyancy, the microbubbles quickly and gently float the dead cells to the surface. Following a centrifugation step, the microbubbles and dead cells are aspirated away leaving the viable cells of interest untouched and ready for downstream applications.

Additional Information:

  • Storage: 4°C
  • Cell Type: Multiple
  • Sample Species and Source: Multiple
  • Cell Separation Method: Negative
  • Capacity: 1 mL of Annexin V BACS™ Microbubbles 
  • Sample Size: 500k-50M cells

This kit comes with:

  • Dead Cell Removal Microbubbles
  • Dead Cell Removal Binding Buffer
  • 1M CaCl2

Recommended for Best Results

  • 20 rpm tube rotator for mixing (e.g., Thermo Scientific cat#: 88881002)
  • Centrifuge (swinging bucket rotor strongly preferred)
  • Vacuum aspirator

Key Features

The Dead Cell Removal Microbubble Kit is designed to deplete apoptotic cells from a variety of biological samples. Unwanted dead and dying cells are captured with Annexin V conjugated BACS™ microbubbles. Captured dead cells are gently removed while enriched cells are left healthy, untouched, and ready for a wide variety of applications such as single-cell techniques, flow sorting, and cell culturing.

Elevated Solutions for Sample Preparation

  • UNCOVER MORE MEANINGFUL BIOLOGY
    Tired of damaged cells? Our gentle workflow isolates cells in their natural state.
  • ENJOY HIGHER RECOVERY
    Keep more cells of interest with over 86% recovery of viable cells to perform more studies to gain greater depth of knowledge.
  • INCREASED VIABILITY
    Get more out of samples that would otherwise be discarded.
  • DO MORE WITH LESS CELLS
    Maximum performance maintained with as few as 500k cells.

Elegant in Simplicity, Powerful in Application

BACS™ Microbubble technology harnesses the power of buoyancy to provide a fast, easy, and gentle cell isolation. Our novel technology couples performance, ease, and quality, bringing cell separation to the next level.

Experience the Benefits of a Microbubble Workflow

  • FREE YOURSELF FROM LIMITATION
    No longer worry about being limited by magnets, losing cells to clogged columns, or purchasing costly equipment
  • ACCOMPLISH MORE
    Save time with a 25-minute protocol, enabling you to focus on your other research
  • SIMPLIFIED WORKFLOW
    Single-tube processing that minimizes handling and loss of target cells

How Microbubbles Compare to Other Methods

  • Flow Sorting: time consuming, expensive, harsh on cells
  • Low Speed Centrifugation: time consuming, low yield
  • Magnetic Beads: low yield, clogged columns, difficult to scale
  • Levitation: low throughput, low capacity, costly equipment

Looking for Answers?

At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and dedicate ourselves to helping researchers obtain exceptional results. If you’re interested in leveraging our BACS™ Microbubble platform to overcome existing hurdles in your cell isolation workflow, we want to hear from you! You can speak directly with an Akadeum scientist available to help you with an optimized workflow. Schedule a call today; we’d love to start a conversation!

Data

Akadeum’s targeted removal of dead cells is achieved through the selective capture of cells with exposed phosphatidylserine (PS) using Annexin V conjugated BACS™ microbubbles. Once mixed with the sample, the BACS Dead Cell Removal microbubbles capture dead cells and float them to the surface for removal, leaving the untouched cells of interest happy, healthy, and ready for downstream use.

Representative Dead Cell Removal

Example of dead cell removal from apopotic mouse splenocytes. Enriched viable cells were stained with DAPI and Annexin V. The fluorescently labeled cells were analyzed by flow cytometry.

Dead Cell Removal Data

Resources

View the Application Note >>

View the Dead Cell Removal Protocol >>

Compare Process Workflows >>

See How Fast The Kit Works In Our Video Demonstration

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