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What Is PBMC? Human PBMC Cells, PBMC Composition, and Immune Cell Processing

Updated on Nov 29, 2023

Peripheral Blood Mononuclear Cells PBMC stands for peripheral blood mononuclear cell. PBMCs are a variety of specialized immune cells that work together to protect our bodies from harmful pathogens. Their location in peripheral blood is significant because they act as a line of defense from infection and disease. PBMCs are typically collected by isolation from whole blood samples.  Where Do PBMCs Come From? PBMCs are developed in the bone marrow …

What is Buffy Coat in Blood? Buffy Coat Preparation and Buffy Coat Cell Extraction

Updated on Nov 28, 2023

What Is a Buffy Coat? In a sample of peripheral whole blood, less than 1% of the cells are white blood cells (WBCs) and platelets. When researchers put the sample through a centrifuge, a machine that spins the blood, those WBCs and platelets combine to form their own layer suspended between the red blood cells (RBCs) and supernatant plasma. This thin layer is called a buffy coat because of its …

Living Cells can React to Magnetic Signals

Updated on Aug 4, 2023

Researchers and scientists around the world have long theorized that the inherent directional systems in various animals (including migratory animals like birds, butterflies, whales, and possibly even humans) could have a magnetic component – that internal navigational impulses are related in some way to Earth’s magnetic field. While this has been a long-standing theory, the underlying cellular mechanisms that support the ability of an organism to detect magnetic field to …

Cell Dissociation Methods for Disaggregation of Tissue: Mechanical vs Enzymatic vs Chemical

Updated on Nov 29, 2023

Cell Dissociation Methods for Disaggregation of Tissue: Mechanical vs Enzymatic vs Chemical

Cell Dissociation Methods Research is undertaken step by step, and cutting corners provides inaccurate results. It’s important to follow proper protocol from beginning to end to ensure the highest quality findings possible. When working with cells of any kind (immune cells, tumor cells, etc.) the first step is always to collect the desired cell sample from the host tissue. Dissociation, sometimes called disaggregation, involves the breaking down of a cell …

Using Akadeum’s Mouse B Cell Isolation Kit to Study Anti-Tumor DNA Vaccines to B Cells as an Alternative to Chemotherapy

Updated on Apr 4, 2023

Use of Akadeum’s gentle Mouse B Cell Isolation kit results in a highly enriched sample of B cells that are healthy and retain their antigen-presenting capabilities. A researcher, Ichwaku Rastogi, from the Doug McNeel Lab at the University of Wisconsin, used the Mouse B Cell Isolation Kit from Akadeum Life Sciences to obtain a high purity isolation of viable, healthy B cells. The researchers are looking into targeted delivery of …

T Cell Immunity & the COVID-19 Vaccine

Updated on Aug 21, 2023

COVID-19 Cells

COVID-19 Reinfection In January 2020, the Center for Disease Control (CDC) confirmed the first domestic case of COVID-19, an infectious disease caused by the SARS-CoV-2 virus. Soon after, COVID-19 was declared a global pandemic, spreading at exponential rates and drastically altering the world as we knew it. After more than a year of research and experiments, we’ve begun to learn more about the mechanisms of this coronavirus variant, and how …

Improving COVID-19 Detection using Saliva Samples instead of NPS

Updated on Aug 4, 2023

  Saliva-based tests can simplify, accelerate, and increase the availability of COVID-19 testing – and potentially lead to better patient outcomes.   The COVID-19 pandemic continues to cause illness across the world, but there is hope on the horizon as improved testing, treatments, and vaccines make their way to the global population. With the emergence of new SARS-CoV-2 variants and mutations, however, it is more important than ever that testing …

COVID-19 Vaccine: How do mRNA Vaccines Work?

Updated on Aug 21, 2023

COVID-19 Vaccine vials

What is an mRNA Vaccine? Messenger ribonucleic acid (mRNA) is genetic material that acts as instructions for cells to develop proteins. An mRNA vaccine harnesses the productive capabilities of mRNA to build up immunities by subjecting the body to proteins that will be recognizable in the future if naturally exposed to a virus. How do mRNA Vaccines Work? While most vaccines contain a weakened or inactive infectious pathogen, mRNA vaccines …

Cell Culture Problems: Cell Clumping – Causes, How to Unclump Cells & How to Avoid Cell Clumping

Updated on Nov 29, 2023

Cell Culture Problems: Cell Clumping – Causes, How to Unclump Cells & How to Avoid Cell Clumping

What Is Cell Clumping? When working with single-suspension cell cultures in a growth medium, it is not uncommon to experience cell loss in a sample. As cells are ruptured, they release DNA and debris that cause cells to aggregate into large clumps that make it difficult for them to expand. Cell clumping can both lead to and be caused by cell apoptosis, or cell death.  As more cells die and …

Cell Harvesting: Methods for Isolating Cell Cultures from a Growth Medium

Updated on Jan 23, 2024

female biologist in a laboratory

Isolating Cell Cultures From a Medium The process of culturing cells involves the introduction of a host population to a growth medium, allowing the population to reproduce quickly in a nutrient-rich environment. After enough cells have spawned to satisfy the requirements of the intended experiment, those same cells need to be removed from the cell culture medium. The cells are intertwined within the substance in which they multiplied and need …

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