Blog Category: General

What is FACS?

March 2020 General

Fluorescence-activated cell sorting (FACS), sometimes called fluorescence-assisted cell sorting, is a specialized type of flow cytometry that uses fluorescent markers to target and isolate cell groups. It is commonly used in hematopoiesis, oncology, and stem cell biology research. How Fluorescence-Activated Cell Sorting Works FACS technology separates cells based on cell surface markers. Antigenic ligands, such as proteins and carbohydrates, give each cell a unique surface phenotype, and specific antibodies associated …

What is the difference between positive and negative cell separation?

March 2020 General

Cell isolation methods and technologies use either a positive or negative cell separation approach. Positive vs. Negative Selection Positive selection involves targeting the desired cell population with an antibody specific to a cell surface marker (CD4, CD8, etc.). The targeted cells are then retained for downstream analysis. Negative selection is when several cell types are removed, leaving the cell type of interest untouched. Similar to positive selection methods, cells are …

How to Isolate Blood Cells from Plasma

March 2020 General

Blood separation is a key part of many life sciences. Researchers in clinical labs and other biotechnical roles commonly analyze blood cells, but to perform this research, whole blood must first be separated into its components. By isolating and enriching target cells from plasma, scientists can then detect diseases, study immunological functions, and further process specific cell types. Components of Whole Blood Whole blood consists of red cells, white cells, …

What is cell isolation?

March 2020 General

Cell isolation—also referred to as cell separation or cell sorting—is the process of isolating one cell population from other cells in a heterogeneous biological sample. Targeted cells are identified, isolated, and then separated according to their type. Examples of Cell Isolation Research based on cell isolation is an essential part of many life sciences, from stem cell and oncology research to routine clinical diagnosis. Cell isolation techniques allow scientists to …

How big are microbubbles?

November 2016 General

Microbubbles range in size from the nanometer length scale to micrometers. The choice in size has a lot to do with the application. For cell separation, it is important that the microbubbles are in something of a Goldilocks Zone, where they can float with a cell attached but not float too quickly. At Akadeum, we have chosen a size of microbubble that is specifically fine tuned for cell separation. In …

What is cell separation and cell sorting?

November 2016 General

We recognize that if you are reading this, you probably already understand or at least have a very good idea of what cell sorting and cell separation is. Still, you would be surprised by how many times we get asked questions like, “What is the difference between cell sorting and cell separation?” We also get questions like, “What are the different ways to separate cells?” or “What is the difference …

What are microbubbles made of?

October 2016 General

When we are out talking about Akadeum’s cell separation microbubbles, we come across a lot of people that think the microbubbles are purely made of a gas. While this is the common type of bubble that many of us are most familiar with, it is not the type of bubble used in separating cells. Akadeum’s microbubbles are made of thin shells of glass with a gaseous core. Microbubbles exist in many different …

How many cells can a microbubble lift?

February 2016 General

We hear this question all the time: How many cells can one of your microbubbles lift? For many users, the most important questions around microbubble-based separation have to do with how many microbubbles to add to a sample. Answering this question requires getting into the total “lifting capacity” of a microbubble. Part of the answer derives from how a microbubble engages cells, clumps of cells, or even larger structures such …