Blog Category: Technical

What Is Flow Cytometry?

August 2020


When researchers handle complex biological samples, they often need to isolate a specific cell population. The process of separating a cell type of interest from others in a heterogeneous mixture, known as cell isolation or cell sorting, allows scientists to perform downstream analysis for life sciences or other research areas. There are many cell sorting methods researchers have at their disposal; one such method is based in flow cytometry. Flow …

What Is MACS?

August 2020


The ability to isolate cell populations from heterogenous mixtures is a key part of life sciences research. One approach to separating cells is magnetic-activated cell sorting (MACS), which uses magnets to isolate targeted cells from the rest of a biological sample. Magnetic cell separation is a popular technology; however, like any method of sorting cells, it has its shortcomings. But MACS needs to look out, because there is a new …

What Are Microbubbles?

July 2020


Microbubbles are small, gas-filled bubbles with applications throughout the life sciences, medicine, and other industries. At Akadeum, we take microbubbles to a new level: Revolutionizing cell separation technology. Akadeum’s buoyancy-activated cell sorting (BACS™) microbubbles are a polydisperse mixture of hollow glass microspheres used to isolate cells in a biological sample. These microbubbles capture target cells and quickly float them to the surface for removal. Our microbubble technology is efficient, gentle, …

What Is FACS?

March 2020


Fluorescence-activated cell sorting (FACS), sometimes called fluorescence-assisted cell sorting, is a specialized type of flow cytometry that uses fluorescent markers to target and isolate cell groups. It is commonly used in hematopoiesis, oncology, and stem cell biology research.

What Is the Difference Between Positive and Negative Cell Separation?

March 2020


Cell isolation methods and technologies use either a positive or negative cell separation approach. Here are some of the differences when considering a cell separation approach:

How to Isolate Blood Cells from Plasma

March 2020


Blood separation is a key part of many life sciences. Researchers in clinical labs and other biotechnical roles commonly analyze blood cells, but to perform this research, whole blood must first be separated into its components. By isolating and enriching target cells from plasma, scientists can then detect diseases, study immunological functions, and further process specific cell types. Components of Whole Blood Whole blood consists of red cells, white cells, …

What Is Cell Isolation?

March 2020


Cell isolation—also referred to as cell separation or cell sorting—is the process of isolating one cell population from other cells in a heterogeneous biological sample. Targeted cells are identified, isolated, and then separated according to their type.

What Is Cell Separation and Cell Sorting?

November 2016


If you are reading this, you probably already understand or at least have a very good idea of what cell sorting and cell separation is. Still, you would be surprised by how many times we get asked questions like, “What is the difference between cell sorting and cell separation?” We also get questions like, “What are the different ways to separate cells?” or “What is the difference between positive and …

Microbubbles and Centrifugation: How It Works

February 2016


Can microbubbles be pelleted by centrifugation? In short, no. At least, not a pellet that you would see at the bottom of your tube when you spin down cells. Here’s how it works.

How Many Cells Can a Microbubble Lift?

February 2016


We hear this question all the time: How many cells can a microbubble lift? For many users, the most important questions around microbubble-based separation have to do with how many microbubbles to add to a sample. Answering this question requires getting into the total lifting capacity of a microbubble. Part of the answer derives from how a microbubble engages cells, clumps of cells, or even larger structures such as spheroids …

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