Publications

Human T Cell Selection, Activation, and Expansion Kit

SKU: 13310-221GMP

Ellis, JS., et al. “Novel Workflow for Activation and Expansion of T Cells Using Microbubble Technology.” The Journal of Immunology 2023; 210(1): 249.16.

Snow, T., et al. “Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles.” JoVE 2022; 190: e64573.

SKU: 13310-221

Grimley, E., et al. “Buoyancy Activated Cell Separation (BACS™) Microbubbles for Lysis and Ficoll Free Processing of Leukopaks.” The Journal of Immunology 2022; 208(1): 173.17.

SKU: 12210-110

Rastogi, I., et al. “B cells require licensing by dendritic cells to serve as primary antigen-presenting cells for plasmid DNA.” Oncoimmunology. 2023; 12(1): 2212550.

This study demonstrates that B cells transcribe and translate antigens encoded by plasmid DNA following passive uptake but require licensing by live Dendritic cells to present antigen to CD8 T cells.
Mouse B cells were used in this study and isolated from splenocytes using Akadeum’s Mouse B Cell Isolation Kit (12210-110)

Jeon, D., et al. “Toll-like receptor agonist combinations augment mouse T-cell anti-tumor immunity via IL-12- and interferon ß-mediated suppression of immune checkpoint receptor expression.” Oncoimmunology. 2022; 11(1): 2054758.

Download the full article here: KONI_11_2054758.pdf (nih.gov)
This study provides a mechanistic rationale for the choice of optimal combinations of TLR ligands to use as adjuvants to improve the efficacy of anti-tumor vaccines.
Mouse B cells were used for this study and enriched from splenocytes of six- to ten-week-old C57BL/6 mice using Akadeum’s Mouse B Cell Isolation Kit (12210-110).

Brooks, J., et al. “Negative feedback by NUR77/Nr4a1 restrains B cell clonal dominance during early T-dependent immune responses” Cell Rep. 2021; 36(9): 109645.

Download the full article here: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564879/pdf/nihms-1737340.pdf (PDF)
This study shows that NUR77 expression scales with antigen stimulation and restrains B cell expansion to preserve early clonal diversity in order to limit holes in the post-immune repertoire and to optimize GC selection.
In this study, mouse B cells were purified using Akadeum’s Mouse B Cell Isolation Kit (12210-110).

SKU: 12210-130

Milne, S., et al. “Myelin oligodendrocyte glycoprotein reactive Th17 cells drive Janus Kinase 1 dependent transcriptional reprogramming in astrocytes and alter cell surface cytokine receptor profiles during experimental autoimmune encephalomyelitis.” Sci Rep. 2024; 14: 13146.

This study provides evidence of Th17 cell reprogramming of astrocytes, which may drive changes in the astrocytic responsiveness to cytokines during autoimmune neuroinflammation.
In this study, mouse CD4+ T cells were isolated from cultured and polarized astrocytes using Akadeum’s Mouse CD4+ T Cell Isolation Kit (12210-130).

Hossain, M.M., et al. “Peripheral-derived regulatory T cells contribute to tumor-mediated immune suppression in a nonredundant manner.” Proc Natl Acad Sci U S A. 2024; 121(36):e2404916121.

This study shows that peripherally-derived Tregs are critical for the early stages of cancer progression and that malignancy can be prevented or delayed by impairing the generation of these cells.
In this study, mouse CD4+ T cells or mouse naive CD4+ T Cells were isolated from fresh or cultured mouse splenocytes using the Mouse CD4+ T Cell Isolation Kit (12210-130) or the Mouse Naive CD4+ T Cell Isolation Kit (12210-150), respectively.

SKU: 11110-000

Fan, C., et al. “Cis -regulatory evolution of the recently expanded Ly49 gene family.” Nat Commun. 2024; 15: 4839.

This study identified a set of cis-regulatory elements (CREs) for activating Ly49genes and shows that in mice, inhibitory and activating Ly49genes are regulated by two separate sets of proximal CREs, likely resulting from lineage-specific losses of CRE activity.
T reg cells were isolated from mouse spleens using Akadeum’s Streptavidin Microbubble Kit (11110-000) and biotinylated CD3 and CD45R antibodies.

Fang, L., et al. “Vascular restoration through local delivery of angiogenic factors stimulates bone regeneration in critical size defects” Bioactive Materials. 2024; 36: 580-594.

This study demonstrates that the local delivery of Spp1 and Cxcl12 enhanced angiogenesis, accelerated bone repair, and mitigated pain responses in mice.
In this study, the regenerative tissues from the defect gap were harvested and dissociated. The erythrocytes were removed using anti-GYPA and Akadeum’s Streptavidin Microbubbles (11110-000)

Lun, M., et al. “Focused x-ray luminescence imaging system for small animals based on a rotary gantry.” J Biomed Opt. 2021; 26(3): 036004.

Download the full article here: JBO-026-036004.pdf (nih.gov) (PDF)
This study demonstrates that the designed FXLT scanner, synthesized nanophosphors, and deep-learning-based reconstruction algorithm show great potential for the high-resolution molecular imaging of small animals.
In this study, Akadeum’s Streptavidin Microbubbles (11110-000) were used for nanophosphors binding in post functionalization steps.

Lun, M., et al. “X-ray luminescence imaging for small animals” Proc SPIE Int Soc Opt Eng. 2020; 11224: 112240F.

Download the full article here: nihms-1555126.pdf (PDF)
This study shows results of building a focused x-ray luminescence tomography (FXLT) imaging system, developing a machine-learning based FXLT reconstruction algorithm, and synthesizing nanophosphors with different emission wavelengths.
In this study, Akadeum’s Streptavidin Microbubbles (11110-000) were used for nanophosphors binding in post functionalization steps.

Part Number: 5610-2214/5610-2214

(Agilent kit commercialized by our partner Agilent Technologies.)

Raynie, D., et al. “New Sample Preparation Products and Accessories for 2024.“ LCGC International 2024; 1(5): 28-33.

Radenovic, S., et al. “Development of a Rapid SARS-CoV-2 Nucleocapsid Assay using Buoyant and Magnetic (BAM) Beads.” 2023; Clemson University 2023; 7.

Wegner, C., et al. “Microbubbles for improved nucleic acid extraction in wastewater samples for viral RNA detection.” medRxiv2022; 7(10): 22277342.