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Share Category: BACS^TM Microbubble Technology and Workflow

Akadeum’s microbubbles represent a breakthrough in process improvement for CAR-T / TCR & other cell therapy processes.

Share Category: BACS^TM Microbubble Technology and Workflow

Working with Microbubbles: Aspiration Best Practices For the most effective microbubble removal, vacuum aspiration is recommended. Here we are using a glass Pasteur pipette to aspirate the microbubble layer. Insert the pipette, bringing it down along the side wall. Aim for the top edge of the microbubble layer where it meets the side of the vial. Carefully vacuum away the microbubble layer, moving the pipette tip around the side wall …

Share Category: BACS^TM Microbubble Technology and Workflow

Akadeum’s Negative Selection cell enrichment kits use streptavidin-coated microbubbles and an optimized cocktail of biotinylated antibodies to label, capture, and remove unwanted cell populations. This workflow takes about half an hour from start to finish, and is exceptionally gentle on delicate immune cells. Using one of Akadeum’s kits for sample preparation can deliver a highly-enriched population of happy, healthy cells for downstream processing.

Share Category: BACS^TM Microbubble Technology and Workflow

Akadeum Life Sciences was founded with the purpose of advancing human health, and in these unprecedented times, we are actively working to contribute to the global scientific community and its understanding of COVID-19 as well as the development of advanced detection capabilities. Automating the microbubble-based nucleic acid extraction workflow To save time, improve throughput, and increase efficiency, it is critical that Akadeum’s nucleic acid extraction microbubble workflow can be automated …

Share Category: BACS^TM Microbubble Technology and Workflow

Magnetic Separation Workflow Many biotech companies are still using magnetic separation, in this process the target cell is captured by a magnetic particle, and a magnetic force pulls the target particles to one side. The amount of force generated depends on the proximity to a rare earth magnet. The non-target cells are removed while the target cells are left behind. A new buffer is added to suspend the magnetic particles. …

Share Category: BACS^TM Microbubble Technology and Workflow

Microbubbles are Fast, Easy, and Gentle! Suspend a sample in up to 50M cells in 100 uL separation buffer Draw up microbubbles with a recommended ratio of 1 microbubble for each target cell Mix bubbles with sample, stir 30 strokes over 1 minute with a standard pipette tip Use a Pasteur pipette to aspirate target cells from the top of the solution Resuspend cells, preparing them for use in a …

Share Category: BACS^TM Microbubble Technology and Workflow

See our Microbubbles in Action! With nothing but a small shake or stir, microbubbles spring into action sorting target cells in a quick and gentle manner. Their small size allows this process to take place in virtually any container.