Akadeum’s Human T Cell Isolation Kit delivers a highly enriched population of healthy, viable T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.
This kit is designed to process 10 samples, up to 100 million cells per sample
Fast & easy workflow enriches human peripheral blood mononuclear cells (PBMCs) for T cells in 30 minutes
No magnet, no column, no harsh chemicals
Microbubble enrichment maintains cell health and physiology
Akadeum’s Human T Cell Isolation Kit delivers a high-purity population of human T cells that are ready for downstream use. Use of these microbubbles to enrich human PBMC samples for T cells prior to flow sorting can greatly reduce final sort times, helping to isolate more cells for better science. Akadeum’s microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of immune cells during enrichment.
Mechanism of Action:Akadeum’s Human T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the T cells of interest while maintaining cellular physiology. The undesired (non-T) cells are labeled with Akadeum’s optimized cocktail of biotinylated antibodies targeting CD14, CD16, CD19, CD36, CD56, CD123, and CD235a. Streptavidin-coated microbubbles are then mixed into the sample, binding to the labeled cells and floating them to the top of the sample container for removal.
How it Works: First, Akadeum’s biotinylated antibody cocktail is added to the sample to label the contaminating cells. Next, the microbubbles are mixed into the sample where the seek out and bind to the unwanted cells. Once bound, they quickly and gently float the undesired cells to the surface. An optional centrifugation step pellets the T cells, and the microbubbles, microbubble-bound cells, and supernatant are easily aspirated away leaving the untouched and enriched T cells happy, healthy, and ready for downstream use.
Additional Information:
Storage: 4°C
Cell Type: T cells
Sample Species and Source: Fresh Human PBMCs
Cell Separation Method: Negative Selection
Capacity: 1 billion cells
This kit comes with:
Akadeum’s BACS™ Streptavidin Microbubbles
Akadeum’s Human T Cell Antibody Cocktail
Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
5mL Eppendorf Tubes
For best results, the following supplies are recommended:
Low retention 1mL pipet tips
Centrifuge with swinging bucket rotor
Vacuum aspirator
Key Features
Akadeum’s Human T Cell Isolation Kit leverages Akadeum’s novel Buoyancy Activated Cell Separation (BACS™) technology for sample preparation that delivers a highly enriched population of healthy, viable T cells using a protocol that is fast, easy, and gentle on cells. Ideal for isolating delicate immune cells, Akadeum’s microbubble enrichment protocol occurs directly in the sample container, eliminating the need to expose cells to harsh chemicals or harmful shear forces from magnetic gradients. The microbubbles are exceptionally gentle and maintain cell health and physiology. T Cell Isolation takes about 30 minutes without the need for additional equipment or expensive consumables like magnets or columns. The end result is a high-purity population of human T cells that are happy, healthy, and ready for downstream use.
Elevated Solutions for Sample Preparation:
Exceptionally gentle T cell enrichment for happy cells
30-minute workflow is fast and easy to complete
No magnet, no column, no extra equipment
Maintain delicate T cell health and physiology
Elegant in Simplicity. Powerful in Application.
Akadeum’s microbubble technology harnesses the power of buoyancy in a fast, easy and gentle workflow without the need for additional equipment. This unique, gentle approach maintains cell health and physiology.
No Magnet. No Column. Happy Cells.
We’ve taken technology that traditionally requires additional equipment to accomplish targeted cell separation and put it into a single container where it’s self-separating. No harmful shear forces, no exposure to external forces like magnetic gradients. Stop losing your precious cells to harsh, outdated processing techniques.
Experience the benefits of a microbubble workflow. Akadeum’s Microbubbles Are:
Magnet-free, protecting your cells from harsh magnetic forces and eliminating the need for additional (and expensive!) equipment
Column-free, using microbubbles directly in the sample vial to separate cells allows for quick and easy isolation of any sample, any volume, anywhere
Gentle on cells, harnessing the targeted efficiency of affinity molecules coupled with the power of buoyancy to gently separate via flotation
Reliable and consistent, delivering fast and easy results that can be scaled for larger volumes with workflows that are compatible with nearly any downstream processing
Looking for Answers?
At Akadeum Life Sciences, our team is committed to your success. We thrive on problem-solving and are dedicated to helping the researchers we work with see exceptional results. If you’re interested in leveraging the microbubble platform to overcome existing hurdles in your sample preparation processes, we want to hear from you! You can speak directly to our team of scientists who are available to help you with an optimized workflow that takes advantage of our microbubble technology and solves long-standing headaches in sample preparation. Schedule a call today, we’d love to start a conversation!
Data
Isolation of human T cells from PBMCs was performed using Akadeum Life Sciences Human T Cell Isolation Kit (#13210-120). Cells were labeled with Streptavidin-FITC and CD3-PE. Dead cells and debris were excluded from analysis.
Resources
Protocol Sheet
How to use Akadeum’s Microbubbles:
FAQs
Cell Therapy Products
What are the minimum and maximum cell numbers that can be processed with Akadeum’s kits?
It is not recommended to process under 10 million cells per sample. The maximum number of cells processed is dependent on the size of the vessel as each kit is very scalable.
What selection method is used for Akadeum’s kits?
Akadeum’s Human T Cell Leukopak Isolation Kit is the first ever negative selection kit that can meet the scale requirements for cell therapy. The Human T Cell Depletion Kit also uses negative selection to deplete endogenous CD3+ T cells. The Human T Cell Selection, Activation, and Expansion Kit uses positive selection.
What is meant by washed leukopak material?
Washed leukopak material refers to a leukopak that has been processed to reduce platelets, exchange buffer, and concentrate the cells. This can be done manually via centrifugation or by using commercially available cell washing instruments.
Can unwashed leukopaks be used with Akadeum’s kits?
Yes, Akadeum has successfully processed raw leukopaks. Please email techsupport@akadeum.com to learn more.
Can Akadeum’s kits be used with cryopreserved samples?
Yes, Akadeum’s kits are designed to work with both fresh and cryopreserved samples.
Are Akadeum’s kits for autologous or allogeneic workflows?
Akadeum’s kits can be integrated into both autologous and allogeneic workflows.
General Questions
What is buoyancy-activated cell sorting (BACS)?
BACS is Akadeum’s proprietary cell separation technology that uses buoyant microbubbles to isolate specific cell populations utilizing only the power of gravity.
What are microbubbles?
Akadeum’s microbubbles are hollow silica spheres that are on average 12 microns.
How big are microbubbles?
Microbubbles average 12 um in diameter. Learn more about microbubbles technology.
How many cells can a microbubble lift?
Microbubbles can carry as many as four cells.
What is the Separation Buffer?
Ca2+, Mg2+ free Dulbecco’s PBS containing 2mM EDTA, and 0.5% bovine serum albumin (BSA). BSA must be biotin free to not interfere with kit performance.
After sitting in the refrigerator, all the microbubbles have separated out of solution. Is there something wrong?
No, this is normal due to the buoyant properties of microbubbles. The solution will need to be mixed well before every use. If processing multiple tubes, mixing between tubes is necessary.
I don’t have an aspirator/vacuum available in my lab, can I still use Akadeum microbubbles?
Aspiration is the best and easiest way to remove the microbubble layer after centrifugation. Pipetting off the bubble layer is not very effective. Vacuum pumps are available from a variety of vendors.
How are Akadeum’s kits shipped?
Kits are shipped on ice packs with overnight delivery.
I have additional technical questions, who should I contact?
Please email all technical questions to techsupport@akadeum.com or check out our chatbot!
