Simple & Fast Removal of Residual RBCs

Akadeum’s Human Red Blood Cell Depletion Microbubbles

Do residual red blood cells interfere with your downstream process? Sample preparation methods can leave unwanted RBCs behind; common sources include:

  • Incomplete RBC lysis
  • Residual RBCs after density gradient
  • High hematocrit remaining in leukopak product

Use Akadeum’s simple Human RBC Depletion Microbubbles protocol to quickly remove residual RBCs

  • Remove assay interference
  • Reduce sort time and improve sort efficiency & cell viability of sorted cells
  • Avoid the effects of cellular debris or magnetic fields on cell phenotype

Simple and Quick Protocol

Effective Removal of Residual RBCs

Red blood cells were spiked into peripheral blood mononuclear cells (PBMCs), the sample was split into two, and one was subsequently subjected to RBC cleanup using Akadeum’s Human Red Blood Cell Depletion Microbubbles Kit. Flow cytometry was performed on the samples and reveals ~97% of RBCs were removed.

Human Red Blood Cell (RBC) Depletion Microbubbles

Cat. 13210-140

  • A great alternative to additional lysis or Ficoll steps
  • No magnets or columns

Product Overview

  • Akadeum’s Human Red Blood Cell Depletion Microbubbles are designed to clean up samples with residual RBC contamination after RBC lysis or Ficoll isolation of human whole blood samples.
  • Microbubbles employ a monoclonal murine lgG against the human RBC glycophorin-A sialoglycoprotein (CD235a). CD235a’s high specificity for erythrocytes, and its high density on RBC surfaces make it an excellent targeting molecule for capture.
  • Upon engaging undesired RBCs, Akadeum microbubbles rapidly lift these cells to the top of the sample for easy removal.

See our App Note on the benefits of Akadeum RBC depletion in cell sorting

Akadeum Human Red Blood Cell Depletion Microbubbles are a simple, effective way to remove unwanted RBCs from samples and can reduce sorting times needed to obtain highly purified cells. Removal of RBCs can be achieved in 10 minutes without the need for any additional equipment. This reduced the time necessary to sort human CD19+ B cells from PMBC samples by one-third.

Additional Data

Akadeum maintains viability and funciton of cell populations after depletion of RBCs

The impact of Akadeum Human Red Blood Cell Depletion Microbubbles on viability (left) and proliferation (right) of a human T cell line (Jurkat) was assessed by spiking in human RBCs, performing an RBC depletion using Akadeum Human Red Blood Cell Depletion Microbubbles, and subsequently determining the viability and cell number at the indicated days after depletion. Trypan blue and a Countess II cell counter were used to evaluate cell number and viability.

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