Do residual red blood cells interfere with your downstream process? Sample preparation methods can leave unwanted RBCs behind; common sources include:
Use Akadeum’s simple Human RBC Depletion Microbubbles protocol to quickly remove residual RBCs
Red blood cells were spiked into peripheral blood mononuclear cells (PBMCs), the sample was split into two, and one was subsequently subjected to RBC cleanup using Akadeum’s Human Red Blood Cell Depletion Microbubbles Kit. Flow cytometry was performed on the samples and reveals ~97% of RBCs were removed.
Akadeum Human Red Blood Cell Depletion Microbubbles are a simple, effective way to remove unwanted RBCs from samples and can reduce sorting times needed to obtain highly purified cells. Removal of RBCs can be achieved in 10 minutes without the need for any additional equipment. This reduced the time necessary to sort human CD19+ B cells from PMBC samples by one-third.
Akadeum maintains viability and funciton of cell populations after depletion of RBCs
The impact of Akadeum Human Red Blood Cell Depletion Microbubbles on viability (left) and proliferation (right) of a human T cell line (Jurkat) was assessed by spiking in human RBCs, performing an RBC depletion using Akadeum Human Red Blood Cell Depletion Microbubbles, and subsequently determining the viability and cell number at the indicated days after depletion. Trypan blue and a Countess II cell counter were used to evaluate cell number and viability.