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New Researcher Spotlight from Harvard: Using Akadeum’s Human CD4+ T Cell Isolation

August 2021

New Researcher Spotlight from Harvard: Using Akadeum’s Human CD4+ T Cell Isolation

A research group led by Professor Georg Lauer at Massachusetts General Hospital and the Harvard Medical School recently used Akadeum’s Human CD4+ T Cell Isolation Kit to obtain high purity enrichment of viable, healthy human CD4+ T cells.

Dr. Hannah Drescher, a postdoctoral associate in the laboratory of Dr. Lauer, used the Akadeum CD4+ T Cell Isolation Kit in the course of their work in understanding the correlates of immune protection from chronic viral infection, the mechanisms employed by viruses to escape host immune surveillance, and to develop immunotherapeutic interventions that resurrect exhausted T cells. The research group has established innovative approaches that allow them to overcome longstanding boundaries of immunological research in humans. They have successfully generated complex direct ex-vivo phenotypic and functional descriptions, together with microarray, RNAseq and ATACseq data from both virus-specific CD4 and CD8 T cells in blood and liver in order to define the specific molecular pathways associated with viral control and immune failure.

In a head-to-head comparison with a magnetic bead-based protocol, the Akadeum protocol delivered 95.4% purity as compared to 86.7% with magnetics. In addition to providing excellent purity, the highly-enriched sample allowed for efficient labeling, detection, and collection of antigen-specific cells of low abundance (specifically CD4+ T cells that recognize HBV-specific epitopes).

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