Microbubbles for Cell Therapy

Akadeum Represents a Breakthrough in Process Improvements for Cell Therapy Manufacturing

Enabling Better Processes to Improve Human Health

Akadeum specializes in the isolation of high value biological targets for downstream use.

Akadeum’s microbubbles can be functionalized to address specific needs in cell therapy workflows, including cell isolation, cell activation, and the depletion of unwanted elements from a sample.

 

 

There is a strong need for efficient, rapid, and gentle sample preparation methods to enable groundbreaking work in cell therapy, and it is critical that these processes can be done at scale – in both concurrent processing as well as processing in larger fluidic volumes.

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Sample preparation is critical to the success of downstream applications, but achieving high-quality results requires that delicate cells of interest be pure and unaltered by harsh retrieval techniques.

Current technologies have failed to meet the needs of evolving scientific research and industries – and that shortcoming is having a negative impact on everything from healthcare costs to research results. 

Akadeum’s revolutionary approach to sample preparation eliminates barriers that are limiting the effectiveness and scalability of workflows today. Our microbubble technology can quickly and easily enrich a complex sample for the analytes of interest while maintaining the health, physiology, and integrity of the enriched sample.

The Next Generation of Separation Technology:
Microbubbles for Cell Therapy Manufacturing

Akadeum’s microbubble technology is helping cell therapy manufacturing overcome longstanding limitations in throughput and scalability.

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The microbubble technology can be deployed in numerous ways for critical workflow elements like:

  • Negative Selection: Enabling High Purity “Leukopak-Scale” T Cell Isolation
  • Positive Selection: Using Akadeum’s Proprietary, Particle-free Protocol
  • Activation: Using Microbubbles to Deliver T Cell Activation Signals
  • Depletion: Removing Unwanted Elements Like Dead Cells from Samples

Microbubbles in Action: T Cell Isolation from Leukopak

Akadeum’s proprietary microbubble workflow can be used for simple T cell isolation from leukopak material. This enables large-volume cell isolation using less equipment and an exceptionally gentle workflow. With the inherent flexibility of the microbubble platform, the separation can be done directly in a bag, or can be done via tube processing.


As shown here, the envisioned process at scale-up allows 10 billion microbubbles to be deployed for cell enrichment that occurs directly in the bag.


These flow plots depict the use of Akadeum’s negative selection protocol to enrich untouched T cells from leukopak material.

  • No pre-processing was required, this isolation occurred directly from the leukopak sample
  • Microbubble enrichment of T cells from leukopak material consistently results in >90% yield

Introducing Akadeum’s Microbubble Platform Technology:
Buoyancy-Based, Single-Container Separation that Scales

Akadeum has developed a novel method to separate cells, proteins, DNA, RNA, and other analytes from a complex biological mixture.

Our proprietary functionalized microbubbles are simply mixed into the sample where they bind to their targets and, leveraging their inherent buoyancy, float the bound targets to the top of the sample container for collection or removal. It’s self-separation in a single container, eliminating common headaches like pour-offs, tube transfers, and processing through volume-constrained columns.

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Akadeum’s Buoyancy-Activated Cell Sorting (BACS™) microbubble technology represents a breakthrough in separation technology.

Microbubbles are small, solid particles that float. They are coated with chemistries that enable them to target specific cells, proteins, nucleic acids, and other analytes of interest. Their elegant mechanism of action allows researchers to leverage the power of gravity for quick and gentle separation that occurs directly in the sample container.

With gravity as the driving force, microbubbles provide consistent results while eliminating the need for extraneous equipment and consumables like magnets and columns, while enabling reliable separations regardless of sample volume or container shape or size.

Akadeum has taken technology that traditionally requires various equipment and consumables to perform, and put it into a single container for self-separation that is exceptionally gentle on delicate cells and other analytes of interest. Critically, this technology works alone or in combination with other cell separation methods or devices, enabling entirely new workflows and assays that are compatible with existing processes.

Elegant in Simplicity, Powerful in Application:
The Akadeum Advantage

Our buoyancy-based approach offers an elegant workflow for floatation-based separation that is exceptionally gentle on valuable analytes while delivering high purity and exceptional yield. The unique mechanism of action enables separation in nearly any volume, from volumes of 10 μL all the way up to liters per assay. This unique scalability enables exceptional throughput, both in terms of fluidic volume being interrogated as well as the number of samples that can be concurrently processed in parallel.

Partnering with Akadeum:
Delivering the Benefits of Microbubble Technology

Akadeum is actively seeking partnerships to provide the benefits of floatation-based target isolation.

Akadeum’s technology benefits partners’ processes with increased throughput, reduced complexity, and superior sensitivity compared to magnetic bead-based extraction. If you are interested in learning more about partnering with Akadeum, you can fill out the form below or contact us at info@akadeum.com. We look forward to hearing from you!


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