Microbubbles for Diagnostics

Akadeum Represents a Breakthrough in Process Improvements for Diagnostics

Enabling Better Processes to Improve Human Health

Akadeum specializes in the isolation of high value biological targets for downstream use.

Akadeum’s microbubbles are a versatile platform technology that can be functionalized to address specific needs in diagnostic workflows, including nucleic acid extraction, circulating tumor cell (CTC) enrichment, wastewater analysis, exosome capture, and more.

 

 

There is a strong need for efficient, rapid, and gentle sample preparation methods to enable effective diagnostic workflows, and it is critical that these processes can be done at scale – in both concurrent (parallel) processing as well as processing in larger fluidic volumes.

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Sample preparation is critical to the success of downstream applications, but achieving high-quality results requires that delicate analytes of interest be pure and unaltered by harsh retrieval techniques, and analytes of low abundance can be collected from within larger-volume, complex sample types.

Current technologies have failed to meet the needs of evolving scientific research and industries – and that shortcoming is having a negative impact on everything from healthcare costs to research results. 

Akadeum’s revolutionary approach to sample preparation eliminates barriers that are limiting the effectiveness and scalability of workflows today. Our microbubble technology can quickly and easily interrogate a complex sample of any volume for the analytes of interest while maintaining the health, physiology, and integrity of the enriched sample.

The Next Generation of Separation Technology:
Microbubbles for Diagnostic Workflows

Akadeum’s microbubble technology is helping diagnostic applications overcome longstanding limitations in throughput and scalability. The microbubble platform can target a wide range of analytes (including cells, nucleic acids, proteins, extracellular vesicles, and more) from a variety of complex biological matrices like blood, tissue, urine, stool, and more.

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Binding Target Cells, Proteins, DNA, and RNA to Microbubbles: Proprietary Manufacturing for Broad Applications

Akadeum’s proprietary technology allows us to functionalize the surface chemistry of the microbubbles for targeted binding to specified analytes. This surface chemistry can range from silica to amine to streptavidin to antibodies and more. Using multiple surface layers allows us to create effective, targeted binding that achieves high yields, minimizes non-specific binding, and creates high purity results for applications like cell separation, pathogen capture, and nucleic acid extraction.

Our proprietary manufacturing system enables high-performance surface functionalization, with shelf life of 1 year already established for multiple targets. All of our manufacturing occurs on-site at Akadeum, where multiple surface layers are used to achieve high yield, low non-specific binding, and high purity for applications like cell separation, pathogen capture, and nucleic acid extraction. Akadeum’s microbubble technology works alone or in combination with other separation methods and devices, and is compatible with nearly any downstream process.

The microbubble technology can be deployed in numerous ways for critical workflow elements like:

  • Negative Selection: Enabling High Purity, Large Scale Enrichment
  • Positive Selection: Extracting Nucleic Acids for qPCR Analysis
  • Depletion: Removing Unwanted Elements Like Dead Cells from Samples

 

Microbubbles in Action: Enabling Enhanced Diagnostic Workflows

Akadeum’s proprietary microbubble technology represents a breakthrough in process improvement for rare target capture from large fluidic volumes. Our technology is enabling new and innovative workflows in diagnostics – everything from nucleic acid extraction to CTC enrichment to wastewater analysis.

Using Microbubbles for Nucleic Acid Extraction

Akadeum’s microbubble platform utility now includes nucleic acid capture protocols. This can be functionalized for nonspecific capture as well as specific sequence capture and can enable streamlined positive selection or depletion protocols.

Learn More about using Microbubbles for Nucleic Acid Extraction >>

CTC Enrichment with Microbubbles

With Akadeum’s microbubbles, depletion of unwanted cell types (like both red and white blood cells) can be easily achieved directly in the sample container to deliver a final sample that is highly enriched for CTCs. In fact, microbubbles can deliver greater than 99% depletion of both RBCs and WBCs, creating a nearly 70-fold enrichment of circulating tumor cells using an exceptionally gentle process that retains >99% of the target tumor cells.

Learn More about using Microbubbles for Liquid Biopsy & CTC Enrichment >>

Combining Microbubbles with Magnetics for Two-Bead Separations

Akadeum’s patented, proprietary approach to leveraging buoyancy in combination with other separation technologies can enable targeted, complex isolations in a single reaction. This allows for the development of streamlined workflows that are easily integrated into existing processes and procedures.

Learn More about Combining Microbubbles with Magnetics for Dual Surface Marker Separation >>

Microbubbles for Wastewater Analysis

Akadeum’s microbubbles are overcoming barriers to rare target capture from complex matrices such as wastewater by using an innovative approach: field-deployable, buoyant, functionalized microbubbles.

Learn More about Wastewater Analysis using Microbubbles >>

Exosome Capture with Microbubbles

Akadeum’s microbubble approach to separation represents a breakthrough in process improvement for rare target capture from large fluidic volumes, and can enable exosome capture from large-volume biological samples. One such example is using functionalized microbubbles for exosome enrichment from urine samples, with the end goal of miRNA isolation for oncology.

Learn More about Exosome Capture with Microbubbles >>

Introducing Akadeum’s Microbubble Platform Technology:
Buoyancy-Based, Single-Container Separation that Scales

Akadeum has developed a novel method to separate cells, proteins, DNA, RNA, and other analytes from a complex biological mixture.

Our proprietary functionalized microbubbles are simply mixed into the sample where they bind to their targets and, leveraging their inherent buoyancy, float the bound targets to the top of the sample container for collection or removal. It’s self-separation in a single container, eliminating common headaches like pour-offs, tube transfers, and processing through volume-constrained columns.

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Akadeum’s Buoyancy-Activated Cell Sorting (BACS™) microbubble technology represents a breakthrough in separation technology.

Microbubbles are small, solid particles that float. They are coated with chemistries that enable them to target specific cells, proteins, nucleic acids, and other analytes of interest. Their elegant mechanism of action allows researchers to leverage the power of gravity for quick and gentle separation that occurs directly in the sample container.

With gravity as the driving force, microbubbles provide consistent results while eliminating the need for extraneous equipment and consumables like magnets and columns, while enabling reliable separations regardless of sample volume or container shape or size.

Akadeum has taken technology that traditionally requires various equipment and consumables to perform, and put it into a single container for self-separation that is exceptionally gentle on delicate cells and other analytes of interest. Critically, this technology works alone or in combination with other cell separation methods or devices, enabling entirely new workflows and assays that are compatible with existing processes.

Elegant in Simplicity, Powerful in Application:
The Akadeum Advantage

Our buoyancy-based approach offers an elegant workflow for floatation-based separation that is exceptionally gentle on valuable analytes while delivering high purity and exceptional yield. The unique mechanism of action enables separation in nearly any volume, from volumes of 10 μL all the way up to liters per assay. This unique scalability enables exceptional throughput, both in terms of fluidic volume being interrogated as well as the number of samples that can be concurrently processed in parallel.

Partnering with Akadeum:
Delivering the Benefits of Microbubble Technology

Akadeum is actively seeking partnerships to provide the benefits of floatation-based target isolation.

Akadeum’s technology benefits partners’ processes with increased throughput, reduced complexity, and superior sensitivity compared to magnetic bead-based extraction. If you are interested in learning more about partnering with Akadeum, you can fill out the form below or contact us at info@akadeum.com. We look forward to hearing from you!


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