Emerging workflows for generating cell-based therapeutics require significantly larger input material than conventional research techniques. This has led to the development of large-scale research strategies based on increased sample sizes. These projects often focus on advancing the collection, processing, and analysis of large quantities of specific cell types and downstream biomedical data.
While large-scale biomedical research is still an emerging player, it has significant implications for complex problems within the field, such as adoptive cell therapy research. As researchers find better ways to collect, process, and analyze large numbers of cells, the results can paint a larger picture to help understand complex cellular phenomena and allow the large-scale production of highly effective cell therapies.
Small-scale cellular research has been the accepted method of biomedical research since the establishment of the field. These projects are spearheaded by single investigators and often focus on smaller goals related to a particular issue within the biomedical field. This is partly due to the limited sample sizes possible with traditional isolation techniques.
In recent years, scientists have seen a greater need to process larger sample sizes to facilitate small-scale research projects. Large-scale and conventional small-scale research methods are both necessary to advance the biomedical science fields, as an advancement in one triggers the development of the other. Nevertheless, small-scale research will continue to be an integral part of the biomedical field, especially as researchers refine the large-scale research method.
Leukopaks are favored over the standard collection of peripheral blood mononuclear cells (PBMC) for large-scale cell research because researchers can extract larger volumes of viable cells from a single donor.
Large-scale purification of cells collected from leukopaks is simplified because the samples contain nearly 14 times more PBMCs.
Leukopak research donor programs can collect samples every 56 days from a single donor, ramping up supply for large-scale cell research projects. Additionally, leukopaks contain a range of cell types, which is beneficial for large-scale researchers concerned with how multiple cell types contribute to a complex biomedical issue.
A single leukopak will contain between six to 20 billion white blood cells, making it an excellent candidate for large-scale cell research. Akadeum’s microbubble technology uses negative selection methods to isolate specific cell types from this complex mix of cells.
The microbubbles bind to unwanted cells, which then rise to the top of the leukopak sample, leaving the cells of interest in the sample’s lower part. Using Akadeum’s Microbubble Leukopak Human T Cell Isolation Kit for leukopak processing eliminates the need for pre-processing samples, thus delivering a larger yield of T cells in a shorter amount of time.
Our kits can provide results in under an hour, allowing researchers to maximize their research efforts.
Traditional leukopak large-scale research protocols involve multiple pre-processing steps, which can result in the degradation of cell quality and reduce the number of viable cells recovered from the sample. Additionally, these methods are time-consuming and often rely on expensive equipment.
With Akadeum’s microbubble technology, leukopak processing for large-scale research has never been simpler. Our technology allows faster processing time using standard lab equipment, reducing the need for costly equipment.
No harsh pre-processing chemicals are used in our kits, thus securing a high yield of cells for each purification cycle without causing unwanted damage to the cells. Researchers can maximize their large-scale cell isolations without sacrificing cost or time.
Large-scale biomedical research is a recent development, with no solid blueprint for researchers to follow. There is also a significant long-term time commitment to developing these projects, making it difficult to secure funding and retain qualified researchers. These projects often rely on interdisciplinary research findings compared to conventional small-scale research endeavors.
Large-scale research requires greater oversight than small-scale research, as the collection of larger samples from human donors is highly regulated. Therefore, strong management and communication between researchers are essential. Financial costs are also higher, as processing and analyzing larger experiments utilize more reagents and can involve equipment with advanced capabilities.
Akadeum wants to save researchers time, resources, and funds by maximizing the yield of purified cells using our microbubble technology. Whether you want to increase throughput for large-scale research projects or conduct conventional small-scale research, our Microbubble Leukopak Human T Cell Isolation Kit can meet your needs.
Are you considering an Akadeum Microbubble Kit for Human T Cell Separation for your next cell separation experiment? Akadeum is happy to answer any questions related to your research efforts.