Working with Microbubbles: Aspiration Best Practices

Working with Microbubbles: Aspiration Best Practices

 

Working with Microbubbles: Aspiration Best Practices

  • For the most effective microbubble removal, vacuum aspiration is recommended. Here we are using a glass Pasteur pipette to aspirate the microbubble layer.
  • Insert the pipette, bringing it down along the side wall. Aim for the top edge of the microbubble layer where it meets the side of the vial.
  • Carefully vacuum away the microbubble layer, moving the pipette tip around the side wall in a circular fashion.
  • Once the microbubbles have been removed, carefully aspirate the separation buffer taking care to leave the cell pellet undisturbed.
  • Resuspend the cell pellet. Cells can be transferred to a new tube, if desired.
  • Your cells are ready for use!

If you have any questions as you work with your microbubble kit, please reach out to our tech support team at techsupport@akadeum.com and we will be happy to help.

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