Microbubbles are Fast, Easy, and Gentle!
- Suspend a sample in up to 50M cells in 100 uL separation buffer
- Draw up microbubbles with a recommended ratio of 1 microbubble for each target cell
- Mix bubbles with sample, stir 30 strokes over 1 minute with a standard pipette tip
- Use a Pasteur pipette to aspirate target cells from the top of the solution
- Resuspend cells, preparing them for use in a total of only 10 minutes