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Microbubble Workflow

Category: BACSTM Microbubble Technology and Workflow

Microbubbles are Fast, Easy, and Gentle!

  • Suspend a sample in up to 50M cells in 100 uL separation buffer
  • Draw up microbubbles with a recommended ratio of 1 microbubble for each target cell
  • Mix bubbles with sample, stir 30 strokes over 1 minute with a standard pipette tip
  • Use a Pasteur pipette to aspirate target cells from the top of the solution
  • Resuspend cells, preparing them for use in a total of only 10 minutes
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