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In Bag Cell Separation with Thermo Fisher Rotea® Compatibility – Akadeum’s Human T Cell Leukopak Isolation Kit – GMP

Category: BACSTM Microbubble Technology and Workflow

Akadeum’s Human T Cell Leukopak Isolation Kit – GMP is uniquely suited for integration into closed, GMP-compliant workflows, including seamless compatibility with the Thermo Fisher Rotea® system. This buoyancy-based cell separation method enables in-bag negative selection, where microbubbles bind unwanted cells and gently float to the top. In contrast, target T cells remain in suspension at the bottom of the bag, ready for downstream processing. The isolated cells can then be harvested directly into a fresh bag using the Rotea®, eliminating open transfers and preserving sample integrity.

Unlike magnetic methods, Akadeum’s microbubble technology does not require proprietary instruments or magnets, making it easier to incorporate into existing cleanroom workflows. The entire process—antibody labeling, microbubble separation, and Rotea cell harvest—takes about 60 minutes and is scalable for clinical and commercial manufacturing environments. See more in the video below.


Integrating Rotea® into Your Workflow?

Discover how Akadeum’s microbubble kits simplify cell separation and boost recovery without magnets or extra equipment.

See the Rotea® Integration Guide →


Akadeum’s microbubble platform enables true flexibility without compromising cell health, viability, or compliance. Explore how this innovative technology supports integrated or instrument-free workflows with our Integration Hub Page, or contact us to request a live demo or customized protocol tailored to your setup.

 

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