Isolating Unique Cell Populations – Quickly, Gently, and Efficiently!

September 2020 Share

Have you run into this problem before?

You’re studying a rare or limited cell population. You prepare your sample and run fluorescence activated cell sorting (FACS™). It’s time consuming. The longer it takes, the more cell death happens. You’re having trouble collecting enough cells for downstream applications. You’re frustrated. You wish there were a better way. A magic wand, perhaps.

You’re not alone.

Sample preparation is critical to the success of downstream applications, but achieving high-quality results requires that samples be pure and unaltered by harsh retrieval techniques. There is a strong need for efficient, rapid, and gentle sample preparation methods to keep up with the advancements in technology for assessing numerous aspects of cellular and molecular biology.

Because current isolation techniques can be time consuming and induce stress on cells, development of a gentle and effective method to obtain a high purity of a small target cell population for downstream analysis is of the utmost importance. That’s where microbubbles from Akadeum come in!

• Microbubbles allow for quick, gentle, and easy enrichment of your sample prior to cell sorting in a process that takes only 30 minutes.
• Using our microbubbles before cell sorting reduces cell sorting times 15-fold and results in highly pure and viable cells that are ready for downstream use.

Ready to learn more? You can access our webinar on-demand at your convenience or download the App Note to review the full experiment and results of combining microbubbles from Akadeum (to enrich the sample quickly and easily) with cell sorting via NanoCellect’s WOLF® Cell Sorter (to gently sort cells while maintaining viability).

It’s not magic, it’s microbubbles!

Akadeum’s Buoyancy-Activated Cell Sorting (BACS™) microbubble technology is a breakthrough in cell separation. Leveraging the power of gravity (the microbubbles float), researchers can achieve quick and gentle removal of captured cells from a complex mixture. Because the microbubbles rely on gravity, they provide reliable and consistent results without needing any additional equipment like magnets or columns. Separation can be achieved regardless of sample volume or container shape or size.

Let’s Connect!

We would love to learn more about your potential applications and discuss whether our microbubbles could be used to improve your workflow. Schedule a 15-minute meeting with one of our technical experts today!