University of Pennsylvania
High Purity Isolation of B Cells Successfully Differentiated to Plasma Cells
Courtesy of: Derek Jones, University of Pennsylvania
key takeaways
- B cell purity > 97%
- Post-enrichment function confirmed
| Objective | Procedure | Outcome |
|---|---|---|
Plasma cell induction after B Cell enrichment |
Spleens: 8-week-old C57BL/6 mice Plasma cell induction: 3-day culture with CpG and IL-5 (bacterial DNA) |
Confirmed upregulation of CD 138 in an enriched population of B Cells |
data
