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High Purity Isolation of B Cells Successfully Differentiated to Plasma Cells

Courtesy of: Derek Jones, University of Pennsylvania

University of Pennsylvania Akadeum Product

key takeaways

  • B cell purity > 97%
  • Post-enrichment function confirmed
Objective Procedure Outcome

Plasma cell induction after B Cell enrichment

Spleens:

8-week-old C57BL/6 mice

Plasma cell induction:

3-day culture with CpG and IL-5

(bacterial DNA)

Confirmed upregulation of CD 138 in an enriched population of B Cells

data

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