University of California, San Francisco
Courtesy of: James Mueller, Julie Zikcherman Lab
B cell purity improved from 36% of non-enriched sample to 81% following fast and easy enrichment. Enrichment was on par with that of Miltenyi’s kit (both viability and purity). Importantly, the BCR percentages were similar to starting material meaning the isolation didn’t disturb these receptors or skew the population which was isolated.
| Objective | Procedure | Outcome |
|---|---|---|
Our laboratory is interested in understanding how autoreactive B cells, despite chronic antigen engagement of the B cell receptor, are restrained from inappropriate activation and differentiation. We are interested in how this process is disrupted in autoimmune disease, and how tolerance mechanisms can be harnessed to re-establish tolerance. |
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