Products

Mouse Red Blood Cell Depletion Kit

The Akadeum RBC Depletion Kit is a microbubble-based cell isolation product designed to clean up samples with significant red blood cell contamination. Common examples include include bone marrow or dissociated tissue samples.

The RBC Depletion kit allows removal of unwanted erythrocytes when harsh lysis steps such as ammonium chloride must be avoided. This is a frequent concern when the RBC lysis conditions may activate, injure, or kill the cells of interest within a sample.

SKU: RM01 Category:

Description

The Akadeum Mouse Red Blood Cell Depletion Kit (Catalog #RB02) provides 2-4 mL containing approximately 180 million Ter119-targeted microbubbles.  This will remove a similar number of total RBCs. Material is shipped in 25mM Tris-buffered saline, pH 7.4, with 0.5% BSA and 0.05% sodium azide as a preservative.

Key Features

High Buoyancy

Upon engaging undesired RBCs, Akadeum microbubbles rapidly lift these cells to the top of the sample (typically less than 10 minutes for passive lifting in a microcentrifuge tube, less than 1 minute with centrifugation).

Excellent Specificity

Microbubbles employ a monoclonal rat IgG2b κ, against the mouse RBC glycophorin-A associated surface protein Ter119 (also known as LY76).  Ter119’s high specificity for erythrocytes and its high density on RBC surfaces make it an excellent targeting molecule for capture.

Fast, Easy Binding in Research Samples

In most samples of volume < 1 mL and total cell number < 108 total cells, mixing with RBC microbubbles can be completed in 1 minute.  Subsequent centrifugation and RBC-bubble removal means the entire clean-up can be accomplished in less than 10 minutes.