Products

Human Red Blood Cell Depletion Kit

$200.00

Why Microbubbles?

  • Up to 99 % capture and removal of RBCs
  • 10 Minute Protocol
  • No Magnet
  • Bubbles float while you are spinning your cells down
  • A great alternative to additional lysis or Ficoll steps

Use microbubbles for

  • Enrichment of circulating tumor cell or other rare cell populations
  • Removal of contaminating RBCs after lysis
  • Removal of contaminating RBCs after Ficoll or equivalent

What’s included

  • 50 million Microbubbles targeted to red blood cells in 1-1.5 mL of reagent
  • Enough microbubbles to remove approximately 50 million RBCs
  • A simple and straightforward protocol
  • Orders of 4 mL or more are discounted. Contact us at info@akadeum.com for more details.
SKU: RB00 Category:

Product Details

The Akadeum Human Red Blood Cell Depletion Kit:

  • 1 mL containing approximately 50 million CD235a-targeted microbubbles.
  • Removes a similar number of total RBCs (1:1 ratio).
  • Reagent is shipped in 25mM Tris-buffered saline, pH 7.4

Key Features

The Akadeum RBC Depletion Kit is a microbubble-based cell isolation product designed to clean up samples with significant red blood cell contamination. Common examples include RBCs that separate poorly in standard Ficoll or leukopheresis preparations. Other examples include bone marrow or dissociated tissue samples.

The RBC Depletion kit allows removal of unwanted erythrocytes when harsh lysis steps such as ammonium chloride must be avoided.  This is a frequent concern when the RBC lysis conditions may activate, injure, or kill the cells of interest within a sample.

High Buoyancy

Upon engaging undesired RBCs, Akadeum microbubbles rapidly lift these cells to the top of the sample (typically less than 10 minutes for passive lifting in a microcentrifuge tube, less than 1 minute with centrifugation).

Excellent Specificity

Microbubbles employ a monoclonal murine IgG against the human RBC glycophorin-A sialoglycoprotein (CD235a).  CD235a’s high specificity for erythrocytes and its high density on RBC surfaces (10^6 per cell, or ~ 7,500 per square micron of RBC surface) make it an excellent targeting molecule for capture.

Fast, Easy Binding in Samples

In most samples of volume < 1 mL and total cell number < 10^8 total cells, mixing with RBC microbubbles can be completed in 1 minute.  Subsequent centrifugation and RBC-bubble removal means the entire clean-up can be accomplished in less than 10 minutes.

Data