Products

Human Leukocyte Depletion Kit (CD45)

The Akadeum WBC Depletion Kit is a microbubble-based cell isolation product designed to clean up samples with undesired white blood cells.

Why Microbubbles?

  • 10 minute depletion protocol
  • Easy workflow
  • No magnet or column needed
Category:

Description

The Akadeum WBC Depletion Kit provides fast and straightforward removal of unwanted cells in your sample.  Material is shipped in 25mM Tris-buffered saline, pH 7.4, with 0.5% BSA and 0.05% sodium azide as a preservative.

Key Features

High Buoyancy

Upon engaging undesired white blood cells, Akadeum microbubbles rapidly lift these cells to the top of the sample (typically less than 10 minutes for passive lifting in a microcentrifuge tube, less than 1 minute with centrifugation).

Excellent Specificity

Microbubbles employ a monoclonal antibody (CD45).  CD45’s high specificity for WBCs and its high density on WBC surfaces make it an excellent targeting molecule for capture.

Fast, Easy Binding in Clinical Samples

In most samples of volume < 1 mL and total cell number < 108 total cells, mixing with CD45 microbubbles can be completed in 1 minute.  Subsequent centrifugation and WBC-bubble removal means the entire clean-up can be accomplished in less than 10 minutes.